Development of an Inorganic-Organic Nano-Assembly Bioimaging Platform

无机-有机纳米组装生物成像平台的开发

• 批准号: 543547-2019
• 负责人: Hemmer, Eva
• 金额: $ 1.82万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=680490
• 关键词: Development; Inorganic; Organic; Nano; Assembly

The development of nanobiotechnology for diagnosis and treatment of diseases addressing current health-related challenges is of great interest for our society. A better understanding of nano-bio-interactions is of paramount importance to design innovative and more efficient solutions for applications such as drug delivery or imaging. Mirexus Biotechnologies Inc (MBI) has developed plant-derived carbohydrate nanoparticles (Phytoglycogen, PhG, derived from sweet corn), so-called NanoDendrix(TM). These are applied in immunomodulation, drug delivery and as anti-infectives. The surface chemistry of these nanoparticles allows for their easy modification to endow the organic nanoparticles with additional functionalities, for instance luminescence due to organic dyes. However, the combination of NanoDendrix(TM) with inorganic nanoparticles has yet to be explored. Of particular interest are lanthanide-based nanoparticles (Ln-NPs), due to their outstanding optical properties based on the use of near-infrared light that is able to penetrate deeper into biological tissues and induces less scattering, phototoxicity, and photobleaching than commonly required UV-vis light. Therefore, this project will develop inorganic-organic nano-assemblies based on NanoDentrix(TM), whose surface will be decorated with sub-10nm sized lanthanide-based nanoparticles. This endows the NanoDentrix(TM) with near-infrared-based optical properties that will be used in order to gain deeper insight into their nano-bio-interaction and ultimately biodistribution. In order to achieve this, we will use the innovative technique of hyperspectral imaging providing environment-specific (e.g. biological cells) optical information. In turn, the knowledge gained will be leveraged for the design and development of more efficient nanoprobes for biomedical applications benefiting Canadian Society.

纳米生物技术用于诊断和治疗当前与健康相关挑战的疾病的诊断和治疗对我们的社会引起了极大的兴趣。更好地了解纳米生物互动对于为药物输送或成像等应用设计创新，更有效的解决方案至关重要。 Mirexus Biotechnologies Inc（MBI）已开发了植物来源的碳水化合物纳米颗粒（源自甜玉米源自甜玉米），所谓的纳米endendrix（TM）。这些应用于免疫调节，药物递送和抗感染物中。这些纳米颗粒的表面化学性质允许其易于修饰，以赋予有机纳米颗粒具有其他功能，例如由于有机染料引起的发光。但是，纳米末端（TM）与无机纳米颗粒的组合尚未探索。特别令人感兴趣的是基于灯笼的纳米颗粒（LN-NP），因为它们的出色光学特性是基于使用近红外光的使用，而近红外光能够深入到生物组织中，并且比常见的散射，光毒性和光漂白更少紫外线光。因此，该项目将基于纳米元素（TM）开发无机有机纳米组件，其表面将用基于灯笼的纳米颗粒进行装饰。这赋予了纳米元素（TM）具有近红外的光学特性，以便深入了解其纳米生物互动和最终生物分布。为了实现这一目标，我们将使用提供环境特异性（例如生物细胞）光学信息的高光谱成像的创新技术。反过来，获得的知识将被利用，用于设计和开发更有效的纳米探针，以使加拿大社会受益的生物医学应用。