Spinning disk confocal microscope for live-cell imaging of pathogen-host cell interactions and biofilm development

用于病原体-宿主细胞相互作用和生物膜发育的活细胞成像的转盘共聚焦显微镜

• 批准号: RTI-2019-00367
• 负责人: Terebiznik, Mauricio
• 金额: $ 10.93万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=666661
• 关键词: Spinning; disk; confocal; microscope; live; Spinning; disk; confocal; microscope; live

Our research groups study highly dynamic cellular and molecular processes occurring as part of the interplay between cells and pathogens, during the immune response and in the formation of biofilms. These processes occur at high speeds, but over periods that can range from several hours or days. To advance our studies, we have an urgent need for a microscope that can capture images of these processes with high spatial and temporal resolution. A microscope of these characteristics is not currently available at the University of Toronto, Scarborough campus. For this reason, we request funding to upgrade a pre-existing Leica DMI6000B inverted microscope into a confocal microscopy system that can fulfill our immediate requirements and long-term research. The proposed upgrade includes: a X-Light V2 Spinning Disk Confocal scanner head to be installed on our Leica DMI6000B inverted microscope, as well as a Light Diode Illuminator with seven laser lines. Importantly, the upgrade will include enclosing the microscope in a Chamlide HX acrylic incubator cage that will provide the appropriate Biosafety containment for live cell experiments with pathogens, toxins and cells. This will also be equipped with on-stage environmental controls, which is crucial for live cell imaging over long periods to ensure cell viability, as well as focal stability for imaging. The requested system will have an immediate positive impact on our research productivity and will substantially improve the training capacity and novel infrastructure exposure for High Qualified Personnel (HQP).**************

我们的研究小组研究了高度动态的细胞和分子过程，作为细胞与病原体之间的相互作用的一部分，在免疫反应和生物膜的形成中。这些过程以高速发生，但在几个小时或几天之间的范围内。为了推进我们的研究，我们迫切需要显微镜，该显微镜可以捕获以高空间和时间分辨率捕获这些过程的图像。当前在士嘉堡校园的多伦多大学尚不提供这些特征的显微镜。因此，我们要求资金升级现有的Leica DMI6000B倒置显微镜为共聚焦显微镜系统，该系统可以满足我们的直接需求和长期研究。拟议的升级包括：要安装在我们的Leica DMI6000B倒置显微镜上的X-Light V2旋转磁盘共聚焦扫描仪头，以及带有七个激光线的光二极管照明器。重要的是，升级将包括将显微镜封闭在Chamlide HX丙烯酸孵化器笼中，该培养箱将为使用病原体，毒素和细胞的活细胞实验提供适当的生物安全遏制。这也将配备阶段的环境控制，这对于长时间的活细胞成像至关重要，以确保细胞活力以及焦点的成像稳定性。所请求的系统将对我们的研究生产率产生直接的积极影响，并将大大改善高素质人员（HQP）的培训能力和新颖的基础设施。**************