Dynamics of the Morphological Features of Microbial Structures in Engineered Environmental Systems

工程环境系统中微生物结构形态特征的动力学

• 批准号: RGPIN-2014-05422
• 负责人: Liss, Steven
• 金额: $ 2.04万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657717
• 关键词: Dynamics; Morphological; Features; Microbial; Structures

2014 will mark the 100th anniversary of the activated sludge process-the dominant technology still applied to biological wastewater treatment. Today, wastewater management issues in Canada and globally include the costs of infrastructure renewal and operations, reducing energy consumption, improving treatment requirements for emerging contaminants, water reuse, energy and resource recovery, and the development and adoption of advanced technologies. Engineers and scientists recognize that new solutions to water management issues will draw upon integration of new tools and advancements in microbiology and biotechnology. **The research proposed employs novel advanced analytical and experimental approaches developed in our laboratory to study the morphological features and physicochemical properties of microbial structures and the communities within. Importantly, the focus is on the dynamic features and changes in structures: as flocs become granules, destabilization of structures including periodic sloughing events in hybrid integrated fixed-film activated sludge systems (IFAS); and the interactions between suspended forms (flocs and granules) and biofilms in the IFAS system. These are relevant not only to understanding fundamental features of these structures but to important design and operating criteria in the application of granular sludge (time to develop granules and their stability) and IFAS reactors (biofilm thickness control and stability). **At a fundamental level the operation of laboratory-scale IFAS reactors permits the opportunity to examine flocs, granules and films in the same reactor operated under well-controlled conditions. Our understanding of the role of extracellular polymeric substances, is improving but incomplete. The roles of proteins, and adhesins in particular, and interactions with polysaccharides in the extracellular polymeric substances of complex microbial communities associated with flocs and films and at microbial interfaces within engineered environmental systems will be explored further. **A key role for the research program is in the development of HQP and the transfer expertise on the application of important new and novel techniques and methods to young trainees and investigators (scientists and engineers) working on environmental problems. Training has been central to the applicant's research program and this will be further enhanced through participation in the NSERC supported CREATE-TWNER (Clean Technologies for Water Refining and Nutrient and Energy Recovery) program.

2014年将纪念活性污泥工艺成立100周年 - 仍然适用于生物废水处理的主要技术。如今，加拿大和全球的废水管理问题包括基础设施更新和运营的成本，减少能源消耗，改善新兴污染物的治疗需求，水再利用，能源和资源回收以及高级技术的开发和采用。 工程师和科学家认识到，对水管理问题的新解决方案将借鉴微生物学和生物技术中的新工具和进步的整合。 **该研究提出，我们在实验室采用了新型的先进分析和实验方法来研究微生物结构和内部社区的形态特征和物理化学特性。重要的是，重点是结构的动态特征和变化：随着泡沫变成颗粒，结构的不稳定，包括混合固定固定胶片中的周期性隔离事件，包括定期链路活动事件（IFAS）；以及IFAS系统中悬浮形式（絮状和颗粒）和生物膜之间的相互作用。这些不仅与了解这些结构的基本特征有关，还与在应用颗粒污泥（发展颗粒及其稳定性的时间）和IFAS反应器（生物膜厚度控制和稳定性）中的重要设计和操作标准有关。 **在基本层面上，实验室规模的IFAS反应堆的运行允许有机会检查在良好控制条件下操作的相同反应堆中的絮凝物，颗粒和膜。 我们对细胞外聚合物物质的作用的理解正在改善但不完整。 将进一步探讨蛋白质，尤其是蛋白质，尤其是蛋白质，以及与群群和膜相关的复杂微生物群落的细胞外聚合物物质以及工程环境系统中的微生物界面的相互作用。 **研究计划的关键作用是在HQP的发展以及将重要新颖的技术和方法应用于年轻的学员和研究人员（科学家和工程师）方面的转移专业知识中。 培训一直是申请人研究计划的核心，这将通过参与NSERC支持的Create-Twner（用于水的清洁技术和养分和能源恢复）计划进一步增强。