Properties and Dynamics of Flocculated, Granular and Fixed Film Microbial Structures in Wastewater Treatment Systems

废水处理系统中絮凝、颗粒和固定膜微生物结构的特性和动态

• 批准号: RGPIN-2020-07224
• 负责人: Liss, Steven
• 金额: $ 2.26万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=718607
• 关键词: Properties; Dynamics; Flocculated; Granular; Fixed

The future of water/wastewater infrastructure must evolve innovatively in response to pressures (e.g. climate change, water quality deterioration, energy/resource costs, inadequate approaches to supporting isolated and remote communities, and urbanization) on legacy systems, and inadequate or ageing infrastructure, as well as opportunities created by the circular economy. The proposed research presents a new perspective to describing, managing and harnessing the properties of biomass within biological wastewater treatment systems. This is important to the changing requirements for effluent treatment, modification and development of new biological treatment systems, migration from large--scale to distributed systems that occupy smaller footprints, and to advance emerging and sustainable technologies. Motivation for the proposed research arises from four emerging insights and developments in our laboratory: (i) A spectrum of microbial structures including floc, granular sludge, granules and fixed-films that can dynamically co-exist and interact in a single stable system, rather than a set of discrete microbial structures; (ii) Aerobic granule systems are not necessarily homogeneous suspensions, and a proportion of the biomass consists of flocculated forms and a range of granular structures representing a yet undefined set of hybrid systems; (iii) Recent observations of rapid granulation in integrated fixed--film sequencing batch reactors and interactions between aerobic granules and fixed-films warrants further study; and, (iv) Unique configurations and microbial interactions in wastewater treatment systems present conditions that contribute to emergence of antimicrobial resistance and mobility of antimicrobial resistance genes in the environment. Our approach employs laboratory-- and pilot--scale bioreactors and examination of fundamental aspects of microbial structures and properties under well--controlled conditions. This includes a recently established novel laboratory--scale continuous flow system designed with features to achieve granulation. Novel methods and advanced tools to further develop insights on the role of extracellular polymers in the properties and behavior of microbial structures in engineered systems. Details of the microbial communities and interactions will involve a combination of molecular based approaches to describe the community composition and dynamics, and the relationship between structural features and microbial interactions. At the core of the research program is training and the development of talent. Our platform includes advanced tools and novel methods that have proven valuable to young trainees and investigators (engineers and scientists) working on environmental problems and at the interface of biology and engineering. Training opportunities will be further enhanced through participation in the NSERC supported CREATE-program - LEaders in wAter anD watERshed Sustainability (the LEADERS).

水/废水基础架构的未来必须根据压力（例如气候变化，水质恶化，能源/资源成本，支持孤立和偏远社区的方法不足，以及对遗产系统的偏远社区的不足以及不足的基础设施或衰老的基础设施以及循环经济创造的机会不足）。 拟议的研究为描述，管理和利用生物量在生物废水处理系统中的特性提供了一种新的观点。这对于不断变化的新生物处理系统的废水处理，修改和开发的需求，从大规模占据到占据较小足迹的分布式系统以及推动新兴和可持续技术的迁移。 拟议研究的动机来自我们实验室中的四个新兴见解和发展：（i）一系列微生物结构，包括泡沫，颗粒状污泥，颗粒和固定膜，它们可以动态共存并在单个稳定系统中相互作用，而不是一组离散的微生物结构； （ii）有氧颗粒系统不一定是均匀的悬浮液，一部分生物质由絮凝形式和一系列代表一组未定义的混合系统组成； （iii）最近观察到综合固定固定粒测序反应堆中快速颗粒的观察结果以及有氧颗粒和固定膜之间的相互作用值得进一步研究； （iv）废水处理系统中的独特构型和微生物相互作用呈现出抗菌抗性的出现和抗菌抗性基因在环境中的迁移率。 我们的方法采用实验室以及试验性生物反应器，并检查在控制良好的条件下微生物结构和特性的基本方面。这包括最近建立的新型实验室 - 规模连续流系统，其设计具有以实现颗粒的特征。新的方法和高级工具，以进一步了解细胞外聚合物在工程系统中微生物结构的性质和行为中的作用。微生物群落和相互作用的细节将涉及基于分子的方法来描述社区组成和动态，以及结构特征与微生物相互作用之间的关系。 研究计划的核心是培训和人才的发展。我们的平台包括先进的工具和新方法，这些方法已证明对年轻的学员和研究人员（工程师和科学家）在生物学和工程的界面上工作。通过参与NSERC支持的创建计划 - 水和流域可持续性（领导者）的领导者，将进一步提高培训机会。