Genetic markers and Bovine Johne's disease

遗传标记和牛约翰氏病

• 批准号: 496214-2016
• 负责人: Karrow, Niel
• 金额: $ 2.7万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=655147
• 关键词: Genetic; markers; Bovine; Johne; disease

Johne's disease (JD) is a disease with great economic implications to the dairy industry and is also attracting**significant public health concern owing to the possible zoonotic nature of its causative bacteria,**Mycobacterium avium subsp. paratuberculosis. The lack of availability of vaccines with high efficacy as well**as effective treatments has hindered control of JD worldwide. Given its heritable nature, as indicated in various**studies, one means of reducing the incidence of JD is by employing genetic selection in breeding programs to**produce offspring with enhanced disease resistance. To achieve this, it is necessary to identify genetic markers**associated with JD and to assess their biological relevance. Over the past decade, a number of genomic studies**have identified different genetic markers associated with JD. Inconsistent findings from these studies suggest**that JD is a complex disease that is polygenic in nature with many potential genetic markers influencing disease**resistance. There is a need to further validate these genetic markers in independent populations before they can**be used in genetic selection programs, and this is the aim of the present study. The specific objectives are to: a)**validate and confirm previous associations with genetic markers and JD in a Canadian Holstein population, b)**assess potential impact of these markers on production and health traits, and c) characterize their biological**relevance in vitro using genetically modified bovine cell lines. New knowledge obtained from this study will**help dairy industry move forward to enhance resistance to JD through genetic selection.

约翰氏病（JD）是一种对乳​​制品行业具有巨大经济影响的疾病，由于其病因细菌可能的人畜共患性质，**雄性分枝杆菌鸟类亚种，也引起了重要的公共卫生关注。旁结核病。由于有效的治疗方法，由于缺乏高效率的疫苗，因此妨碍了全球JD的控制。鉴于在各种**研究中所表明的那样，降低JD发病率的一种方法是在育种计划中采用遗传选择来**产生具有增强疾病耐药性的后代。为了实现这一目标，有必要识别与JD相关的遗传标记，并评估其生物学相关性。在过去的十年中，许多基因组研究**已经确定了与JD相关的不同遗传标记。这些研究的发现不一致地表明，JD是一种复杂的疾病，本质上是多基因的，具有许多潜在的遗传标志物影响疾病**抗性。需要在遗传选择程序中使用这些遗传标记，以进一步验证这些遗传标记，这是本研究的目的。具体目标是：a）**验证并确认在加拿大荷斯坦人口中与遗传标记和JD的先前关联，b）** **评估这些标志物对生产和健康特征的潜在影响，c）使用遗传改良的牛油细胞系在体外表征其生物学**相关性。从这项研究中获得的新知识将**通过遗传选择来帮助乳制品行业前进，以增强对JD的抵抗力。