Identification and Characterization of Novel Molecular Markers for the Proteobacteria (Beta and Gamma) and Firmicutes Phyla

变形菌门（β 和 γ）和厚壁菌门新型分子标记的鉴定和表征

• 批准号: 249924-2012
• 负责人: Gupta, Radhey
• 金额: $ 2.48万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=652709
• 关键词: Identification; Characterization; Novel; Molecular; Markers

Microorganisms (i.e. bacteria) play a central role in all aspects of life including recycling of organic materials, nitrogen fixation, photosynthesis, waste decomposition, and in human health and diseases. Therefore, it is of much importance to clearly distinguish different groups of microbes and determine what unique properties they possess. Although the cultured "Bacteria" are presently divided into about 25 main groups, for most of these groups no unique molecular, biochemical or physiological characteristics are known. Hence, it is of much importance to identify characteristics that are unique to different groups of bacteria. Using genome sequences, my group has pioneered the discovery of two types of molecular markers (i) conserved inserts or deletions (indels) in protein sequences and (ii) whole proteins that are specific for different bacterial lineages. These markers in addition to providing highly specific means for identification of different groups of bacteria also provide valuable probes for genetic and biochemical studies. This proposal will extend our extensive earlier work in a number of important ways. Our main objectives are: **1. Undertake detailed comparative analyses of genome sequences from the Proteobacteria and Firmicutes phyla to identify molecular markers that are specific for them. The Proteobacteria and Firmicutes are two of the largest groups within bacteria containing numerous organisms of industrial, clinical, agricultural and environmental interest. Hence, the discovered molecular markers will be useful for a variety of studies.**2. Use the newly discovered molecular markers to develop more sensitive and specific methods for identification of a number of important bacterial groups (e.g. Streptococcus, Listeria, Xylella, Pectobacterium, Dickeya, Ralstonia, Neisseria, etc.) that are part of these phyla.**3. Perform genetic and biochemical studies on a number of molecular markers to understand their functional significance. In this regard, functional studies will be carried out on a number of conserved indels in important housekeeping proteins (viz. Gyrase A, Gyrase B, DnaK and SecA).**********

微生物（即细菌）在生命的各个方面发挥着核心作用，包括有机材料的回收、固氮、光合作用、废物分解以及人类健康和疾病。因此，清楚区分不同的微生物群体并确定它们具有哪些独特的特性非常重要。尽管培养的“细菌”目前分为约 25 个主要类群，但对于这些类群中的大多数，尚无独特的分子、生化或生理特征。因此，识别不同细菌群的独特特征非常重要。利用基因组序列，我的团队率先发现了两种类型的分子标记（i）蛋白质序列中的保守插入或删除（indels）和（ii）针对不同细菌谱系的完整蛋白质。这些标记除了提供识别不同细菌群的高度特异性手段之外，还为遗传和生化研究提供有价值的探针。该提案将以多种重要方式扩展我们早期的广泛工作。我们的主要目标是：**1。对变形菌门和厚壁菌门的基因组序列进行详细的比较分析，以确定它们特有的分子标记。变形菌门和厚壁菌门是细菌中最大的两个类群，含有大量具有工业、临床、农业和环境意义的生物体。因此，所发现的分子标记将可用于各种研究。**2。使用新发现的分子标记开发更灵敏和更特异的方法来鉴定属于这些门的许多重要细菌群（例如链球菌、李斯特菌、木杆菌、果杆菌、迪克氏菌、拉尔斯顿氏菌、奈瑟菌等）。 ** 3.对许多分子标记进行遗传和生化研究，以了解它们的功能意义。在这方面，将对重要管家蛋白（即旋转酶 A、旋转酶 B、DnaK 和 SecA）中的一些保守插入缺失进行功能研究。 **********