Identification and Characterization of Novel Molecular Markers for the Proteobacteria (Beta and Gamma) and Firmicutes Phyla

变形菌门（β 和 γ）和厚壁菌门新型分子标记的鉴定和表征

• 批准号: 249924-2012
• 负责人: Gupta, Radhey
• 金额: $ 2.48万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=506035
• 关键词: Identification; Characterization; Novel; Molecular; Markers

Microorganisms (i.e. bacteria) play a central role in all aspects of life including recycling of organic materials, nitrogen fixation, photosynthesis, waste decomposition, and in human health and diseases. Therefore, it is of much importance to clearly distinguish different groups of microbes and determine what unique properties they possess. Although the cultured "Bacteria" are presently divided into about 25 main groups, for most of these groups no unique molecular, biochemical or physiological characteristics are known. Hence, it is of much importance to identify characteristics that are unique to different groups of bacteria. Using genome sequences, my group has pioneered the discovery of two types of molecular markers (i) conserved inserts or deletions (indels) in protein sequences and (ii) whole proteins that are specific for different bacterial lineages. These markers in addition to providing highly specific means for identification of different groups of bacteria also provide valuable probes for genetic and biochemical studies. This proposal will extend our extensive earlier work in a number of important ways. Our main objectives are:

微生物（即细菌）在生活的各个方面都起着核心作用，包括对有机材料的回收，氮固定，光合作用，废物分解以及人类健康和疾病。因此，清楚区分不同的微生物组并确定它们所拥有的独特特性非常重要。尽管目前将培养的“细菌”分为大约25个主要群体，但对于大多数组，尚无独特的分子，生化或生理特征。因此，确定不同细菌群是独有的特征非常重要。使用基因组序列，我的小组率先发现了两种类型的分子标记物（i）蛋白质序列中的保守插入或缺失（indels），以及（ii）针对不同细菌谱系的全蛋白。除了提供高度特异性的方法以鉴定不同群体的细菌外，这些标记还为遗传和生化研究提供了有价值的探针。该提案将以多种重要方式扩展我们的早期工作。我们的主要目标是：