Bioactive filtrate recovery from autothermal thermophilic aerobic digestion (ATAD) to produce microbial-derived plant stimulants

从自热嗜热需氧消化 (ATAD) 中回收生物活性滤液以生产微生物来源的植物兴奋剂

• 批准号: 531139-2018
• 负责人: Eskicioglu, Cigdem
• 金额: $ 1.82万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=650296
• 关键词: Bioactive; filtrate; recovery; autothermal; thermophilic

TerraBioGen, previously known as International Bio Recovery Corporation, is an agricultural biotech company**that develops cell-free, bio-stimulants and microbial metabolite bio-products that encourage plant growth and**enhance their tolerance to biotic and abiotic stresses. In the past, TerraBioGen operated an autothermal**thermophilic aerobic digestion (ATAD) facility in which it processed organic municipal solid waste (mostly**food waste) from the City of Vancouver and produced Class A biosolids fertilizer. The large-scale (i.e., six 60**ton and three 120 ton) digesters operated by TerraBioGen were able to produce bioactive fractions successful at**achieving ATAD conditions. These bioactive fractions contained naturally occurring bacterial strains that also**had promising effects on plant growth, such as enhanced root development, improved plant health, increased**tolerance against biotic and abiotic stresses and enriched crop yield. Since termination of the large-scale**ATAD, TerraBioGen tried to produce similar bioactive fractions using small-scale digesters (i.e., 1500, 100,**and 10 L) but was unsuccessful. Hence, TerraBioGen has an urgent need of developing similar biostimulants in**lab-scale digesters to maintain production at high level of quality. This project aims to explore the basic steps**involved in developing process and operating conditions at a lab-scale ATAD that encourages the microbial**community observed at large-scale. Laboratory experiments will be conducted at the Bioreactor Technology**Group Laboratory. ATAD will be simulated by automated fermenters with dissolved oxygen (DO), pH and**temperature probes. As substrate, food waste from cafeterias on UBC's Okanagan Campus will be used. If**results from the lab-scale experiments are promising, TerraBioGen will be able to generate bioactive fractions**and save significant capital and operational cost related to building and operating large scale ATAD digesters.**By using the biostimulant, the stress on current agricultural practices for farming more land and more irrigation**and using more fertilizer to meet increasing demand will be reduced. The project will train highly qualified**personnel in areas of waste reduction and resource recovery.

TerraBioGen 以前称为国际生物恢复公司，是一家农业生物技术公司**，开发无细胞生物刺激剂和微生物代谢生物产品，促进植物生长并**增强植物对生物和非生物胁迫的耐受性。过去，TerraBioGen 运营着一个自热**嗜热需氧消化 (ATAD) 设施，在其中处理来自温哥华市的有机城市固体废物（主要是**食品废物）并生产 A 级生物固体肥料。 TerraBioGen 运营的大型（即六个 60** 吨和三个 120 吨）消化池能够成功生产达到 ATAD 条件的生物活性组分。这些生物活性部分含有天然存在的细菌菌株，这些菌株对植物生长也具有良好的效果，例如增强根系发育、改善植物健康、增加**对生物和非生物胁迫的耐受性以及提高作物产量。自从大规模 **ATAD 终止以来，TerraBioGen 尝试使用小型消化器（即 1500、100、**和 10 L）生产类似的生物活性组分，但没有成功。因此，TerraBioGen 迫切需要在实验室规模的消化器中开发类似的生物刺激剂，以保持高水平的生产。该项目旨在探索实验室规模 ATAD 开发工艺和操作条件所涉及的基本步骤**，以鼓励大规模观察微生物**群落。实验室实验将在生物反应器技术**组实验室进行。 ATAD 将通过带有溶解氧 (DO)、pH 和**温度探头的自动发酵罐进行模拟。作为基质，将使用 UBC 奥肯那根校区自助餐厅的食物垃圾。如果**实验室规模实验的结果有希望，TerraBioGen 将能够生成生物活性组分**并节省与建造和运营大型 ATAD 消化器相关的大量资金和运营成本。**通过使用生物刺激剂，压力当前耕种更多土地和更多灌溉**以及使用更多化肥以满足不断增长的需求的农业做法将减少。该项目将培训废物减少和资源回收领域的高素质人才。