Expanding the use of high-speed sCMOS cameras into selective 3-dimensional imaging

将高速 sCMOS 相机的使用扩展到选择性 3 维成像

基本信息

批准号：
515504-2017
负责人：
Yethiraj, Anand
金额：
$ 1.82万
依托单位：
Memorial University of Newfoundland
依托单位国家：
加拿大
项目类别：
Engage Grants Program
财政年份：
2017
资助国家：
加拿大
起止时间：
2017-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639282
关键词：
Expanding use speed sCMOS cameras

项目摘要

High-speed cameras have provided unprecedented ability to look at biological live-cell processes in real time aswell as machine vision in manufacturing processes. In both cases, one obtains two-dimensional image series ofthree-dimensional objects. Accessing real-time information outside a two-dimensional (X-Y) plane, i.e., in X,Y, and Z dimensions, is a challenge. Typical confocal scanning microscopes address the problem ofvisualization in 3 dimensions. However, such microscopes are expensive (of order $500,000) and, nevertheless,have limited capabilities for processes that involve high-speed out-of-plane motions.Accessing real-time information in three dimensions has been a challenge that our commercial partner, OptikonCorporation Ltd., is keen to resolve. To solve this problem, the proposed collaborative work aims to establish aprocedure that uses known components - a scientific CMOS camera (sCMOS), along with high speed focusingoptics - to get 3D information without requiring true confocal scanning. The end point of this proposal wouldbe a Technical Application Note that enables future purchasers of sCMOS cameras from the commercialpartner, Optikon Corporation Ltd., to have access to clear guidelines on how to integrate high-speed focusingoptics with a sCMOS camera in order to be able to visualize multiple regions in X,Y and Z in real time.

高速摄像机提供了前所未有的能力，可以实时查看生物活细胞工艺以及制造过程中的机器视觉。在这两种情况下，都可以获得三维图的二维图像序列。访问二维（X-Y）平面（即X，Y和Z维度）之外的实时信息是一个挑战。典型的共聚焦扫描显微镜在3个维度中解决了视觉化的问题。但是，这样的显微镜价格昂贵（订单$ 500,000），但是，对于涉及高速平面运动动作的流程的功能有限。在三个维度上获得实时信息是我们的商业合作伙伴OptikonCorporation Ltd.的挑战。为了解决这个问题，拟议的协作工作旨在建立使用已知组件（科学CMOS摄像机（SCMOS））以及高速焦点焦点 - 以获取3D信息而无需真正共同扫描的情况。该提案的终点将是一项技术申请说明，该说明可以从商业公司Optikon Corporation Ltd.的未来购买者进行SCMOS摄像机的未来购买者，可以访问有关如何与SCMOS摄像机集成高速焦点相机的明确准则，以便能够在x，y和z中可视化x，y和z的多个区域。