Surface spectroscopy and microscopy studies of molecular films at electrified interfaces

带电界面分子膜的表面光谱和显微镜研究

• 批准号: RGPIN-2016-03958
• 负责人: Lipkowski, Jacek
• 金额: $ 7.61万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=632465
• 关键词: Surface; spectroscopy; microscopy; studies; molecular

The general objective of this project is to study the structure and reactivity of functional monolayers and bilayers of phospholipids deposited onto a metal electrode at the metal-solution interface. The functionality of these films will be determined by the presence of peptides and proteins. Our goal is to learn how the electric field affects the stability of mixed bilayers composed of phospholipids and cholesterol, incorporation of proteins into the bilayer and electron and ion transfer through proteins incorporated into the supported bilayer. To achieve these goals, we will employ electrochemical methods, scanning probe microscopies such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM), in situ photon polarization modulation Fourier transform infrared reflection absorption spectroscopy (PM-IRRAS), surface enhanced infrared and Raman spectroscopies and the neutron scattering technique. We will use electrochemical techniques to control the physical state of the film and the scanning probe microscopies to image the field driven transformations of the membranes. In addition, spectroscopic and neutron scattering techniques will be employed to study conformational changes of organic molecules and their ordering within the membrane. Our goal is to obtain fundamental understanding of several biorelevant processes such as:(i) interactions of antimicrobial (AMPs) and amyloid peptides with phospholipid bilayers, (ii) interactions of monolayers and bilayers of nucleolipids with complementary nucleobases in solution, (iii) conformational and orientational changes of blue copper oxidase enzymes occurring during oxidation/reduction of their redox center. The metal electrode surface, covered by a biomimetic membrane, can be charged and electric fields on the order of 10^7 – 10^8V/m can be applied to the membrane. These fields have comparable magnitude to the fields acting on biological membranes. Our goal is to use this unique opportunity to manipulate biomolecules with the field and to monitor conformational and orientational changes induced by the filed using the surface analytical techniques. In addition, for the membrane supported at a metal electrode, the electrochemical methods could be used for transduction of analytical information about the host guest interactions in the membrane to an electrical signal for analytical detection.

该项目的一般目标是研究沉积在金属溶剂界面上金属电子上的磷脂的功能单层和双层的结构和反应性。这些薄膜的功能将取决于辣椒和蛋白质的存在。我们的目标是了解电场如何影响由磷脂和胆固醇组成的混合双层的稳定性，将蛋白质掺入双层中，并通过蛋白质和离子转移通过掺入支持的双层的蛋白质和离子转移。为了实现这些目标，我们将采用电化学方法，扫描探针显微镜，例如扫描隧道显微镜（STM）和原子力显微镜（AFM），原位光子光子极化傅立叶傅立叶变换转化感染感染反射分析光谱（PM-IRRRAS），表面增强的基础和拉曼等级和中性群体和中等性的散射技术。我们将使用电化学技术来控制膜的物理状态和扫描探针显微镜，以对膜的场驱动转换进行图像。此外，光谱和中子散射技术将被采用来研究有机分子的构象变化及其在膜内的序列。我们的目标是获得对几个生物含量过程的基本理解，例如：（i）抗菌（AMP）和淀粉样蛋白肽与磷脂双层的相互作用，（ii）单层与氧化酶酶的核氧化酶酶的相互作用在其氧化氧化酶降低过程中发生在其氧化氧化氧化氧化氧化氧化氧化酶的过程中。金属电极表面被仿生膜覆盖，可以为10^7 - 10^8v/m的阶充电，并可以将电场应用于膜。这些磁场与作用于生物膜的田地具有可比性。我们的目标是利用这一独特的机会来操纵现场的生物分子，并监视使用表面分析技术提出的宪法和定向变化。此外，对于在金属电极支撑的膜，电化学方法可用于翻译有关膜中宿主客人相互作用到电信号的分析信息，以进行分析检测。