Genetic Mechanisms Underlying Quantitative Traits in Maize - Dosage Effects & Loss of Function Mutations

玉米数量性状的遗传机制——剂量效应

• 批准号: RGPIN-2014-05673
• 负责人: Lee, Elizabeth
• 金额: $ 1.89万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=588553
• 关键词: Genetic; Mechanisms; Underlying; Quantitative; Traits

Loss of gene function is a mechanism by which quantitatively inherited traits respond to selection is the underlying premise of this DG research proposal. This premise was built on observations made while working with a maize Zea mays L.) population under long-term selection, builds upon my group’s previous research, and continues the examination of genetic architecture underlying quantitative traits in maize. Three distinct yet interconnected hypotheses will be examined in this proposal. They are distinct in that they involve very different traits – starch synthesis, ear development, leaf number and flowering time. They are connected in that they involve single-gene loss of function alleles and are potentially involved in altering meristem development. Specifically: (1) The short-ear phenotype was achieved by accumulating & maintaining a set of LOF alleles for genes involved in ear development in a heterozygous state thus effectively reducing the level of expression of these genes (i.e., gene dosage) and resulting in a short-ear phenotype. (2) Modifier of amylose extender 1 (mae1) is a LOF mutation that confers a moderately higher level of amylose. (3) Selection for an altered shoot apical meristem (SAM) based relationship between leaf number and flowering time will be achieved through the accumulation of LOF mutations. Impact of this research is both in the applied and basic knowledge arenas. It will serve as a training ground for 2 M.Sc. students, 1 Ph.D. student, and 5 undergraduate students. And it will lead to the development of novel intellectual property in the form of maize germplasm exhibiting altered SAM based relationship between leaf number and flowering time.

基因功能的丧失是对选择的机械性遗传性状的反应，这是该DG研究的基本前提。该前提是基于与玉米蛋白的Zea Mays L.在长期选择下进行的观察结果。 ，并继续研究玉米中遗传结构的基本定量特征。 三个独特的假设将在它们中进行研究，它们涉及非常nt的，耳朵发育，叶子数和开花时间在杂合状态下涉及耳朵发育的基因的LOF等位基因有效地降低了对透过基因的水平（即基因剂量），并导致基于短耳的表型。 LOF突变的积累。 两种基础知识领域的影响。