Sustainable Bioprocessing Approaches for Large-Scale Jadomycin Production

大规模生产雅多霉素的可持续生物加工方法

• 批准号: 341213-2013
• 负责人: Brooks, MarianneSuLing
• 金额: $ 1.6万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=572197
• 关键词: Sustainable; Bioprocessing; Approaches; Large; Scale

Antimicrobial resistance is a serious public health issue, driving the urgent need for novel drugs. The most successful drug discovery route uses natural products from microbes and chemically modifies them. Promising derivatives then undergo animal/clinical trials, however, obtaining sufficient material from fermentations is an issue. Jadomycins show potential as novel therapeutic agents. When Streptomyces venezuelae bacteria are stressed and supplied with amino acids, different jadomycins are produced, providing a source of molecular scaffolds for creating drugs. The jadomycin process has several stages: (1) growth of bacteria, (2) application of environmental stress, (3) jadomycin production and (4) downstream processing. Our overall research goal is the development of sustainable fermentation technologies for optimal large-scale production of jadomycins. The proposed approach includes the evaluation of by-products from other industries (glycerol from biodiesel production and amino acids from fish processing) as components in microbial growth and production media for optimal jadomycin production. The combined use of glycerol and fish by-products in the jadomycin production process is an original and innovative way to: (1) enhance the economic and environmental sustainability of the jadomycin process and (2) improve the economic and environmental impact of the biodiesel and fish processing industries. The development of a fish-derived amino acid mixture for use in the jadomycin production media will allow a novel procedure to be developed where several jadomycins can be produced from a single fermentation. Continuous large-scale production of jadomycins will have industrial significance via decreased downtime and improved product consistency. This research would benefit all Canadians through the development of a novel drug and efficient production methods. It will also enable several Highly Qualified Personnel (HQP) to be trained.

抗生素耐药性是一个严重的公共卫生问题，推动了对新药的迫切需求。 最成功的药物发现途径是使用微生物的天然产物并对其进行化学修饰。有前途的衍生物随后进行动物/临床试验，然而，从发酵中获得足够的材料是一个问题。 Jadomycins 显示出作为新型治疗剂的潜力。 当委内瑞拉链霉菌受到压力并提供氨基酸时，会产生不同的贾多霉素，为制造药物提供分子支架来源。 贾多霉素工艺有几个阶段：(1) 细菌生长，(2) 环境胁迫的应用，(3) 贾多霉素生产和 (4) 下游加工。 我们的总体研究目标是开发可持续发酵技术，以实现贾多霉素的最佳大规模生产。 拟议的方法包括评估其他行业的副产品（生物柴油生产中的甘油和鱼类加工中的氨基酸）作为微生物生长和生产培养基的成分，以实现最佳贾多霉素生产。 在贾多霉素生产过程中联合使用甘油和鱼副产品是一种原创和创新的方法，可以：(1) 增强贾多霉素工艺的经济和环境可持续性；(2) 改善生物柴油和生物柴油的经济和环境影响。鱼类加工业。 用于贾多霉素生产培养基的鱼源氨基酸混合物的开发将允许开发一种新的程序，其中可以通过单次发酵生产多种贾多霉素。 贾多霉素的连续大规模生产将通过减少停机时间和提高产品一致性而具有工业意义。 这项研究将通过开发新型药物和高效的生产方法使所有加拿大人受益。 它还将使数名高素质人员（HQP）得到培训。