Develop quantitative standards for microbial mercury-processing enzymes

制定微生物汞处理酶的定量标准

• 批准号: 461945-2013
• 负责人: Poulain, Alexandre
• 金额: $ 1.82万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=542826
• 关键词: Develop; quantitative; standards; microbial; mercury

Mercury (Hg) is a top priority contaminant and a potent neurotoxin in its methylated form. Microbes are key players in the Hg cycle and can be harnessed for bioremediation purposes. Environmental Proteomics (EP) specializes in producing commercial antibodies and calibrated quantitation standards for use in environmental science, and has designed these tools for processes such as photosynthesis, carbon and nitrogen fixation, heat shock and stress responses. With its continued focus on key environmental biochemistry, EP aims to expand its reach into bacteria-mediated processes such as metal detoxification, and to market the products and detection services internationally. Studies of biological Hg transformations require sensitive approaches for measuring not only the different species of Hg, but also the microbial mediators of Hg detoxification. Proteins are the functional molecules of the cell and direct measurements of Hg processing proteins are a powerful and needed addition to research on microbial Hg detoxification pathways. The primary objective of this project is to

汞 (Hg) 是一种首要污染物，其甲基化形式是一种强效神经毒素。微生物是汞循环的关键参与者，可用于生物修复目的。环境蛋白质组学 (EP) 专门生产用于环境科学的商业抗体和校准定量标准品，并为光合作用、碳和氮固定、热休克和应激反应等过程设计了这些工具。 EP 持续关注关键环境生物化学，旨在将其业务范围扩大到金属解毒等细菌介导的过程，并将产品和检测服务推向国际。生物汞转化的研究需要灵敏的方法，不仅可以测量不同种类的汞，还可以测量汞解毒的微生物介质。蛋白质是细胞的功能分子，直接测量汞处理蛋白是微生物汞解毒途径研究的有力且必要的补充。该项目的主要目标是