Regulatory mechanisms of Na+ and fluid absorption across alveolar epithelium

肺泡上皮钠和液体吸收的调节机制

• 批准号: 312177-2011
• 负责人: Brochiero, Emmanuelle
• 金额: $ 2.19万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=526419
• 关键词: Regulatory; mechanisms; Na+; fluid; absorption

Effective gas exchange in the lungs requires alveolar air spaces virtually free of liquid. It has been established that liquid clearance from alveoli is mainly driven by active Na+ transport through ENaC channels, located at the apical membrane of alveolar epithelial cells. The regulatory mechanisms of ENaC as well as Na+ and fluid absorption across alveolar cells are complex and remain incompletely understood. Since 2005, our research program thus aims at better understanding the mechanisms that control alveolar Na+ and liquid absorption. During the last few years, we have made some significant progress by identifying several crucial regulatory elements of alveolar transport. Indeed, we have identified several factors including the activity of another class of ion channels, the K+ channels, controlling ENaC gene promoter activity and ENaC expression, as well as alveolar Na+ and liquid transport.

肺部有效的气体交换需要肺泡空气空间几乎没有液体。已经确定，肺泡的液体清除主要是由通过位于肺泡上皮细胞顶膜的 ENaC 通道的活性 Na+ 转运驱动的。 ENaC 以及肺泡细胞的 Na+ 和液体吸收的调节机制很复杂，目前仍不完全清楚。因此，自 2005 年以来，我们的研究计划旨在更好地了解控制肺泡 Na+ 和液体吸收的机制。在过去的几年中，我们通过确定肺泡运输的几个关键调控元件取得了一些重大进展。事实上，我们已经确定了几个因素，包括另一类离子通道 K+ 通道的活性，控制 ENaC 基因启动子活性和 ENaC 表达，以及肺泡 Na+ 和液体转运。