Isothermal titration calorimeter

等温滴定量热仪

• 批准号: 439731-2013
• 负责人: Epand, Richard
• 金额: $ 7.53万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=505644
• 关键词: Isothermal; titration; calorimeter

The funds will be used to purchase an isothermal titration calorimeter (ITC). An ITC is an instrument that measures the heat absorbed or released as a consequence of mixing two substances. Since virtually all processes are accompanied by a change in heat, the useful of this method is extremely broad. At McMaster University there are biochemists, chemical biologists, biophysicists and chemical engineers planning to use this method. An ITC instrument has one component placed in a motor-driven syringe and the other component is placed in the cell of the calorimeter and maintained at a very constant temperature. Small fixed volumes from the syringe are delivered at a predetermined rate to the calorimeter cell. This results in heat being absorbed or released. It can be determined how many times material can be delivered before there is no further change in heat flow (except for a small effect resulting from the heat of dilution). From the series of injections one can calculate both the heat change in the reaction as well as the affinity of the two substances for each other. The process can be performed at a range of temperatures to understand how the reaction varies with temperature. The method will be applied to a broad range of problems including the binding of small molecules to large biopolymers and to understanding the absorbitive properties of membrane interfaces. The results will be used to explain the basis of phenomena observed by other methods. Applications will develop for understanding how to optimize interactions with drug targets and how to protect drug carriers and biomaterials from absorbing other substances. There are a total of 10 principle invesitgators on this application. Application of ITC will advance the research of each participant. It will allow accurate, quantitative date to be obtained. Advances will occur in a number of areas including basic biology, biomedical engineering and chemistry. It will allow the training of individuals with high technical skill to contribute to Canadian science, both in acedemia as well as industry.

这些资金将用于购买等温滴定热量计（ITC）。 ITC是一种仪器，可测量由于混合两种物质而导致的吸收或释放的热量。由于几乎所有过程都伴随着热量的变化，因此该方法的有用非常广泛。麦克马斯特大学（McMaster University）有生物化学家，化学生物学家，生物物理学家和化学工程师计划使用这种方法。 ITC仪器的一个组件放在电动机驱动的注射器中，另一个组件放置在量热计细胞中，并保持在非常恒定的温度下。注射器的小固定体积以预定速率传递到量热计细胞。这导致热量吸收或释放。可以确定在没有进一步变化的热流之前可以输送多少次材料（除了稀释热引起的效果很小）。从一系列注射中，人们可以计算反应的热变化以及两种物质的亲和力彼此的亲和力。该过程可以在一系列温度下进行，以了解反应如何随温度变化。该方法将应用于广泛的问题，包括小分子与大型生物聚合物的结合，并了解膜界面的吸收特性。结果将用于解释其他方法观察到的现象的基础。将开发应用程序，以了解如何优化与药物靶标的相互作用，以及如何保护药物携带者和生物材料免受吸收其他物质。该应用程序中总共有10个主要的入侵者。 ITC的应用将推进每个参与者的研究。它将允许获得准确的定量日期。进步将在许多领域中发生，包括基本生物学，生物医学工程和化学。这将允许对具有高技术技能的人进行培训，以在流动性和行业中为加拿大科学做出贡献。