Refrigerated Centrifuge for Small Volume Applications

适用于小体积应用的冷冻离心机

• 批准号: 422314-2012
• 负责人: MacRae, Thomas
• 金额: $ 0.66万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495768
• 关键词: Refrigerated; Centrifuge; Small; Volume; Applications

The instrument requested in this application will be used for the centrifugation, under controlled temperature, of small samples (1.5 ml or less) containing DNA, RNA and protein. This is a routine but essential procedure for the applicant, his students and others working in the Department of Biology who will have access to the centrifuge. Gene expression and protein synthesis during diapause, a physiological state of dormancy and enhanced stress tolerance, will be studied in embryos of the crustacean, Artemia franciscana. The DNA, RNA and proteins prepared from Artemia are used in several functional assays. Protein structure/function relationships and the contribution of molecular chaperones, which protect and fold other proteins, to cell survival during dormancy are important components of the work. The refrigerated centrifuge will therefore be used to make fundamental contributions to cell, molecular and developmental biology. In addition, there are many applications for the research. Examination of diapause may reveal how insects resist stress, this of importance in agriculture and forestry where the impact of these organisms as pests is exacerbated by their ability to enter diapause and survive harsh environmental conditions. A greater understanding of diapause will enhance the use of Artemia as feed in aquaculture. Stress-induced augmentation of the molecular chaperone HSP70 in Artemia larvae increases their resistance to infection by bacteria, an observation with potential importance in the development of new procedures for the control of disease in commercially important aquatic invertebrate species. The role of HSP27, a small heat shock protein, in breast cancer cell metastasis will be studied. Clearly there is potential to make discoveries which have direct consequences to commerce and health through use of a refrigerated microcentrifuge. The equipment will also facilitate experiments addressing basic questions which are the cornerstone of research programs supported by NSERC. Funding this modest proposal will put the applicant at a definite advantage in the effective use of grant funds and the ability to conduct modern experiments in several biological disciplines.

本申请中要求的仪器将用于在受控温度下离心含有 DNA、RNA 和蛋白质的小样品（1.5 ml 或更少）。对于申请人、他的学生和在生物系工作的其他可以使用离心机的人来说，这是常规但必不可少的程序。将在甲壳类卤虫的胚胎中研究滞育期间的基因表达和蛋白质合成，滞育是一种休眠和增强的应激耐受性的生理状态。从卤虫中制备的 DNA、RNA 和蛋白质可用于多种功能测定。 蛋白质结构/功能关系以及保护和折叠其他蛋白质的分子伴侣对休眠期间细胞存活的贡献是这项工作的重要组成部分。因此，冷冻离心机将用于为细胞、分子和发育生物学做出根本性贡献。此外，该研究还有许多应用。滞育检查可能揭示昆虫如何抵抗压力，这在农业和林业中很重要，因为这些生物体作为害虫的影响因其进入滞育和在恶劣环境条件下生存的能力而加剧。对滞育的更深入了解将促进卤虫作为水产养殖饲料的使用。卤虫幼虫中应激诱导的分子伴侣 HSP70 增强增强了它们对细菌感染的抵抗力，这一观察结果对于开发控制商业上重要的水生无脊椎动物物种疾病的新程序具有潜在的重要性。将研究HSP27（一种小型热休克蛋白）在乳腺癌细胞转移中的作用。显然，通过使用冷冻微量离心机有可能发现对商业和健康产生直接影响的发现。该设备还将促进解决基本问题的实验，这些问题是 NSERC 支持的研究项目的基石。资助这项规模不大的提案将使申请人在有效利用资助资金以及在多个生物学科进行现代实验的能力方面具有明确的优势。