Incubator shaker and accessories

培养摇床及配件

• 批准号: 407542-2011
• 负责人: MacRae, Thomas
• 金额: $ 1.76万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472655
• 关键词: Incubator; shaker; accessories

The instrumentation will be used primarily for the controlled culture of bacteria, generally Escherichia coli. This is an essential procedure for the researcher submitting the application and for others working in the Department of Biology who will have access to the incubator shaker and accessories. Cultured bacteria will be used in cloning cDNAs encoding proteins up-regulated during diapause, and for expression of wild type and mutated eukaryotic proteins which are then purified. These proteins are used in several in vitro assays to investigate the properties of proteins such as molecular chaperones and as antigens for production of antibodies used in protein quantification and localization. The incubator shaker will be used to grow Artemia under controlled temperature and aeration. Gene expression and protein synthesis during Artemia embryo development and diapause, a state of physiological dormancy and enhanced stress tolerance, will be studied. Protein structure/function relationships and the contribution of molecular chaperones to cell survival during diapause are important components of this work. The research employing the incubator shaker will make fundamental contributions to cell, molecular and developmental biology, and there are many applications of the work. Examination of diapause may reveal how insects resist stress, this of importance in agriculture and forestry where the impact of these organisms as pests is exacerbated by the ability to enter diapause and survive harsh environmental conditions. A greater understanding of diapause will enhance the use of Artemia as feed in aquaculture. Dr. MacRae studies the role of HSP27, a small heat shock protein, in breast cancer cell metastasis. Clearly there is potential to make discoveries which have direct consequences to commerce and health through use of the incubator shaker and accessories sought herein. The equipment will also facilitate experiemnts addressing basic questions which are the cornerstone of research programs supported by NSERC. Funding this proposal will put the applicant at a definite advantage in the effective use of grant funds and the ability to conduct modern experimentation in several biological disciplines.

该仪器将主要用于细菌（通常是大肠杆菌）的受控培养。对于提交申请的研究人员和生物系的其他工作人员来说，这是一个重要的程序，他们可以使用培养箱摇床和配件。培养的细菌将用于克隆编码滞育期间上调蛋白质的cDNA，并用于表达野生型和突变的真核蛋白质，然后将其纯化。这些蛋白质被用于多种体外测定中，以研究蛋白质的特性，例如分子伴侣，以及作为生产用于蛋白质定量和定位的抗体的抗原。培养箱摇床将用于在受控温度和通气条件下培养卤虫。将研究卤虫胚胎发育和滞育（一种生理休眠和增强的应激耐受性状态）期间的基因表达和蛋白质合成。蛋白质结构/功能关系以及分子伴侣对滞育期间细胞存活的贡献是这项工作的重要组成部分。使用培养箱摇床的研究将对细胞、分子和发育生物学做出基础性贡献，并且该工作有许多应用。对滞育的检查可能揭示昆虫如何抵抗压力，这在农业和林业中很重要，因为这些生物体作为害虫的影响因进入滞育并在恶劣的环境条件下生存的能力而加剧。对滞育的更深入了解将促进卤虫作为水产养殖饲料的使用。 MacRae 博士研究 HSP27（一种小型热休克蛋白）在乳腺癌细胞转移中的作用。显然，通过使用本文寻求的培养箱摇床和配件，有可能做出对商业和健康产生直接影响的发现。该设备还将促进解决基本问题的实验，这些问题是 NSERC 支持的研究项目的基石。资助该提案将使申请人在有效利用资助资金以及在多个生物学科进行现代实验的能力方面具有明确的优势。