Role of yeast-mycelium transition in the pathogenicity of the Dutch elm disease fungus Ophiostoma novo-ulmi

酵母菌丝体转变在荷兰榆树病真菌 Ophiostoma novo-ulmi 致病性中的作用

• 批准号: 105519-2008
• 负责人: Bernier, Louis
• 金额: $ 3.72万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=475311
• 关键词: Role; yeast; mycelium; transition; pathogenicity

Dutch elm disease (DED) is the most destructive disease of elms. It has been estimated that, during the last 100 years, over one billion mature elms were killed by two successive pandemics of DED caused by two microscopic fungi called Ophiostoma ulmi and O. novo-ulmi. Our ongoing research suggests that an Ophiostoma gene (called Omp2) coding for a high affinity ammonium transporter is important for the virulence of the DED pathogens towards elms. We intend to clarify the role of Omp2 in pathogenesis. In particular, we will verify the hypothesis that the action of Omp2 stems from its effect on the regulation of yeast and mycelium forms of the DED fungi in the presence of low concentrations of ammonium. We further propose to analyse two additional genes which may be involved in other regulatory pathways for fungal growth, as suggested by the recovery of O. novo-ulmi mutants with impaired yeast or mycelial development. The short-term benefit of this project is a better understanding of how the DED fungi develop in elms and eventually kill them. Anticipated long-term benefits are the identification of fungal targets for the development of efficient and environmentally acceptable control methods to save the elms.

荷兰榆树病（DED）是榆树的最具破坏性疾病。据估计，在过去的100年中，超过10亿个成熟的榆树被两种连续的DED杀死，由两种称为Ophiostoma ulmi和O. Novo-Ulmi的微观真菌引起的DED造成。我们正在进行的研究表明，编码高亲和力转运蛋白编码的腹瘤基因（称为OMP2）对于DED病原体对ELMS的毒力很重要。我们打算阐明OMP2在发病机理中的作用。特别是，我们将验证以下假设：OMP2的作用源于其在低浓度铵的情况下对DED真菌的调节的影响。我们进一步建议分析可能参与其他调节性真菌生长途径的其他基因，这是通过恢复酵母菌或菌丝体发育受损的Novo-ulmi突变体的建议。该项目的短期利益是更好地了解DED真菌如何在ELMS中发展并最终杀死它们。预期的长期福利是确定真菌目标，以开发有效且环保的控制方法以节省榆树。