Discovery Projects - Grant ID: DP210100362

发现项目 - 拨款 ID：DP210100362

• 批准号: ARC : DP210100362
• 负责人: Miguel Valvano
• 金额: $ 53.45万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/discovery-projects-grant-id-dp210100362/1782711
• 关键词: Discovery; Projects; Grant; ID; DP210100362; Discovery; Projects; Grant; ID; DP210100362

Characterising O-linked glycosylation across Burkholderia. Protein glycosylation, the chemical addition of sugars to proteins, enables the augmentation of protein properties. Across the Burkholderia genus we have shown O-linked glycosylation is both conserved as well as essential for bacterial fitness. Yet, we have little understanding of how glycosylation modulates the proteome of this genus. This project aims to characterise the glycoproteomes of Burkholderia species and track the impact of glycosylation on both the proteome and protein stability. By understanding how glycosylation shapes the proteome we will gain a greater understanding of the role of bacterial glycosylation in Burkholderia physiology as well as how we may better utilise microbial glycosylation for glycoprotein production.

表征整个Burkholderia的O连锁糖基化。蛋白质糖基化是将糖化学成蛋白质的化学添加，从而增强了蛋白质特性。在整个Burkholderia属中，我们已经显示O连接的糖基化既保守，也是细菌适应性的必不可少的。但是，我们对糖基化如何调节该属的蛋白质组几乎没有理解。该项目旨在表征Burkholderia物种的糖蛋白酶，并跟踪糖基化对蛋白质组和蛋白质稳定性的影响。通过了解糖基化如何塑造蛋白质组，我们将对细菌糖基化在Burkholderia生理学中的作用有更深入的了解，以及我们如何更好地利用微生物糖基化来产生糖蛋白。