Burkholderia species in sugarcane: the relationship among antifungal production, intrinsic antimicrobial resistance, and pest biocontrol

甘蔗中的伯克霍尔德氏菌：抗真菌生产、内在抗菌素耐药性和害虫生物防治之间的关系

• 批准号: BB/R022607/1
• 负责人: Miguel Valvano
• 金额: $ 8.53万
• 依托单位: Queen's University Belfast
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR022607%2F1
• 关键词: Burkholderia; species; sugarcane; relationship; among

The Burkholderiaceae comprises a family of environmental bacteria widespread around the planet, which reside in diverse environments and in association with plants, insects, animals, and humans and also display extraordinarily high multidrug, intrinsic antibiotic resistance. Intrinsic resistance means that these bacteria can tolerate large concentrations of many different classes of antibiotics. Burkholderia are very useful microorganisms for mankind. They can serve as agents for bioremediation, plant growth promotion, and pest biocontrol, but these beneficial properties remain virtually unexploitable because of fear of infection and antibiotic resistance. Indeed, some Burkholderia species are particularly dangerous to humans and domestic animals (e.g. melioidosis and glanders) and they are singled out as bioterrorism agents, while others (e.g. B. cenocepacia and B. multivorans) cause debilitating and often transmissible lung infections in cystic fibrosis patients. Burkholderia spp. is a main bacterium of the sugarcane cultivable bacterial community in Brazil, and can control fungal and infections by producing many different antifungal compounds. Research in our laboratories show that strains with high-level antimicrobial resistance and increased bacterial virulence in a widely used insect infection model (Galleria mellonella) display higher capacity to inhibit sugarcane pathogenic fungi. These observations suggest that antimicrobial resistance and antifungal production are interrelated. We hypothesise that antimicrobial resistance and antifungal compound production are coordinated to allow Burkholderia survival in the presence of a polymicrobial flora in the plants and also to defend and complete for nutrients against other organisms in the rhizosphere. Our proposal aims to exploit this idea to find new ways to maximise the beneficial properties of Burkholderia for use in the field (e.g. antifungal production) while reducing antimicrobial resistance in the clinic. We will combine molecular microbiology, genomics, and infection models in plants and insects to assess the hypothesis that antifungal production drives antimicrobial resistance by influencing the production of molecules that enhance bacterial cell envelope impermeability resulting in increased antibiotic resistance, which in turn increases bacterial virulence. We will also exploit these findings to develop novel ways to inhibit antimicrobial resistance and enhance pest biocontrol.

Burkholderiaceae包括在地球周围广泛的环境细菌家族，该家族驻留在多种环境中，并与植物，昆虫，动物和人类结合，并且还表现出非常高的多药，内在的固有抗生素耐药性。固有的抗性意味着这些细菌可以忍受许多不同类别的抗生素。 Burkholderia是人类非常有用的微生物。它们可以用作生物修复，植物生长促进和有害生物控制的药物，但是由于担心感染和抗生素耐药性，这些有益的特性几乎仍然无法解释。实际上，一些伯克霍尔德物种对人类和家畜特别危险（例如Melioidisos和腺体），它们被视为生物恐怖剂，而另一些物种则被视为生物恐怖剂，而其他（例如B. Cenocepacia and B. Multivorans）会导致衰弱，并且经常会导致静脉纤维化病患者的肺部感染。 Burkholderia spp。是巴西甘蔗可栽培细菌群落的主要细菌，可以通过产生许多不同的抗真菌化合物来控制真菌和感染。在我们的实验室中的研究表明，在广泛使用的昆虫感染模型（Galleria Mellonella）中，具有高级抗菌耐药性并增加细菌毒力的菌株显示出更高的能力抑制甘蔗致病真菌。这些观察结果表明，抗菌耐药性和抗真菌性产生相互关联。我们假设抗菌素耐药性和抗真菌化合物的产生是协调的，以使植物中有多数菌群菌群的存在，并为根茎中的其他生物提供养分和完整。我们的建议旨在利用这一想法，以找到新的方法来最大程度地提高Burkholderia在该领域使用的有益特性（例如抗真菌生产），同时降低诊所中的抗菌素耐药性。我们将在植物和昆虫中结合分子微生物学，基因组学和感染模型，以评估抗真菌生产通过影响分子的产生来增强细菌细胞膜包膜无素不舒张能力，从而促进抗菌抗生素的抗生素耐药性，从而促进抗菌细胞的耐药性，从而促进了抗生素的抗生素抗性，从而促进了抗生素的抗生素，从而促进了抗生素的耐药性。我们还将利用这些发现来开发新的方法来抑制抗菌耐药性并增强害虫生物防治。

项目成果

Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction.

• DOI: 10.1016/j.micres.2020.126451
• 发表时间: 2020-02
• 期刊: Microbiological research
• 影响因子: 6.7
• 作者: Karent J Romero-Gutiérrez;M. N. Dourado;L. M. Garrido;L. R. Olchanheski;E. T. Mano;F. Dini‐Andreote;M. Valvano;W. L. Araújo