Effect of environmental stress conditions on functional and structural properties of photosystem II

环境胁迫条件对光系统II功能和结构特性的影响

基本信息

批准号：
93404-2007
负责人：
Popovic, Radovan
金额：
$ 2.77万
依托单位：
Université du Québec à Montréal
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2010
资助国家：
加拿大
起止时间：
2010-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=451217
关键词：
Effect environmental stress conditions functional

项目摘要

The regulation of Photosystem II structure and electron transport activity is strongly affected by environmental factors such as light and the presence of different pollutants which cause inhibitory effects. In this research project, we attempt to understand in which way structure and functions are accommodated to cope with those environmental conditions. When the photosynthetic apparatus is exposed to toxic effects induced by heavy metals and herbicides the excess light energy not used in photochemistry will be dissipated via pigments of the light harvesting complex through thermal dissipation or formation of reactive oxygen species. Our hypothesis is that, under those conditions, the mechanism of energy dissipation will reflect the specificity of the inhibition mechanism induced by heavy metals and herbicides. In our project, by using different species of higher plant and alga and their specific mutants, we will investigate the mechanism of energy dissipation process associated with Photosystem II. Our research objective is to study the relationships between the effects of inhibitors, heavy metals and herbicides induced under strong light intensity and energy dissipation mechanisms. By using highly advanced spectroscopic studies and biochemical methods, experiments will be done in in vitro and in vivo conditions. Results obtained will provide information which can explain how plants can survive and tolerate pollutants toxicity effects under different light conditions. These results will contribute to fundamental knowledge concerning the interaction between inhibitory effects of some pollutants and light energy dissipation mechanism.

光系统II结构和电子传递活性的调节受到环境因素的强烈影响，例如光和不同污染物的存在，从而产生抑制作用。在这个研究项目中，我们试图了解结构和功能以何种方式适应这些环境条件。当光合装置受到重金属和除草剂引起的毒性作用时，光化学中未使用的多余光能将通过热耗散或活性氧物质的形成，通过光捕获复合体的色素耗散。我们的假设是，在这些条件下，能量耗散机制将反映重金属和除草剂引起的抑制机制的特异性。在我们的项目中，我们将通过使用不同种类的高等植物和藻类及其特定突变体，研究与光系统II相关的能量耗散过程的机制。我们的研究目标是研究抑制剂、重金属和除草剂在强光强度下诱导的效应与能量耗散机制之间的关系。通过使用高度先进的光谱研究和生化方法，将在体外和体内条件下进行实验。获得的结果将提供解释植物如何在不同光照条件下生存并耐受污染物毒性作用的信息。这些结果将有助于了解一些污染物的抑制作用与光能耗散机制之间的相互作用的基础知识。