Responses of roots to signals from environmentally perturbed shoots

根对环境干扰芽信号的反应

• 批准号: 5727-2007
• 负责人: Reid, David
• 金额: $ 3.06万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=449847
• 关键词: Responses; roots; signals; environmentally; perturbed

My long-term interests are: (i) What are the mechanisms by which plants sense & respond to environmental stress/change. (ii) What is the role of hormones & other signal molecules as messengers & amplifiers of a fluctuating environment? (iii) How do shoots communicate with roots & roots communicate with shoots? Environmental change/stress triggers the production of multiple signals & these acting in concert modify growth & development in ways that increase survival. Roots & shoots are trapped in dissimilar environments, yet both organs must act cooperatively. Above- & below-ground organs need to efficiently exchange information about conditions being experienced by the other distant organ. What processes do these messages trigger when they reach the target organ? What is the identity of messages passing between organs in response to sensing of environmental alteration? How are such messages coordinated? How do the networks of interacting messages function? In this proposal I will focus on the question, what happens in roots when they receive messages/signals from environmentally perturbed shoots? Roots make up approximately 50% of the world's terrestrial plant biomass yet there is surprisingly little research on roots compared to aboveground tissues. Being buried in the soil presents technical challenges. While investigation of roots is fraught with difficulties, we have developed techniques that allow us to effectively probe the physiology & biochemistry of this inadequately understood organ. The hypothesis being tested is: Shoots, subjected to range of environmental perturbations (different red/far red irradiation, UV-B, wind stress, high CO2) will send signals to roots triggering them to quickly modify their metabolism & growth & development. Some of the parameters to be measured are: changes in levels of root hormones (concentrating on ethylene in the first instance), metabolic fingerprinting & footprinting of roots & root exudates, & monitoring of root architecture & morphology with MacRhizo software.

我的长期兴趣是：（i）植物感知和响应环境压力/变化的机制是什么。 (ii) 激素和其他信号分子作为波动环境的信使和放大器的作用是什么？ (iii) 芽如何与根沟通以及根如何与芽沟通？环境变化/压力会触发多种信号的产生，这些信号协同作用，以提高生存的方式改变生长和发育。根和芽处于不同的环境中，但两个器官必须协同运作。地上和地下器官需要有效地交换有关其他远处器官所经历的状况的信息。当这些信息到达目标器官时会触发哪些过程？器官之间响应环境变化而传递的信息的身份是什么？这些消息是如何协调的？交互消息网络如何发挥作用？在这个提案中，我将重点关注这个问题：当根部收到来自环境扰动的芽的信息/信号时，会发生什么？根部约占世界陆地植物生物量的 50%，但与地上组织相比，对根部的研究却少得惊人。埋在土壤中提出了技术挑战。虽然对根的研究充满了困难，但我们已经开发出技术，使我们能够有效地探索这个尚未充分了解的器官的生理学和生物化学。正在测试的假设是：受到一系列环境扰动（不同的红光/远红光照射、UV-B、风胁迫、高二氧化碳）的芽将向根部发送信号，触发它们快速改变新陈代谢、生长和发育。要测量的一些参数包括：根激素水平的变化（首先集中于乙烯）、根和根分泌物的代谢指纹和足迹，以及使用 MacRhizo 软件监测根结构和形态。