Portable nanofluidic aptamer-SERS instrument for measurement of chemical exposure

用于测量化学暴露的便携式纳米流体适体-SERS 仪器

基本信息

批准号：
8981629
负责人：
George W Jackson
金额：
$ 71.42万
依托单位：
BIOTEX, INC.
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-19 至 2018-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8981629
关键词：
Address Analytical Chemistry Base Pairing Biochemical Biological Biological Assay Biological Monitoring Biotechnology Blood Blood Circulation Blood Tests Blood specimen Budgets Carcinogens Chemical Exposure Chemicals Chemistry Chromatography DNA Detection Devices Disease Engineering Environment Environmental Exposure Environmental Health Enzyme-Linked Immunosorbent Assay Exposure to Fluorescence Free Will Generations Goals Gold Hand Health Professional Housing Human Individual Laboratories Lasers Ligands Light Liquid substance Mass Spectrum Analysis Measurement Measures Methodology Microfluidic Microchips Microfluidics Modeling Monitor Nanotechnology Optics Patients Performance Phase Physicians Poison Polychlorinated Biphenyls Publishing Raman Spectrum Analysis Reporter Reporting Research Research Design Research Personnel Risk Sampling Sensitivity and Specificity Side Signal Transduction Sister Small Business Innovation Research Grant Source Specificity Spottings Surface System Systems Analysis Technology Testing Texas Text United States National Institutes of Health Universities aptamer base bisphenol A blood filtration clinical application constriction cost effective detector environmental agent exposed human population improved innovation instrument instrumentation mass spectrometer molecular recognition nanochannel nanofluidic novel phase 2 study phthalates point of care product development professor programs public health relevance response stressor

项目摘要

DESCRIPTION (provided by applicant): The goal of this NIH-SBIR Phase II effort is to develop a new point-of-care (POC) detection platform and methodology for assessment of human exposure to hazardous environmental compounds in the bloodstream. Because the mechanisms of transport of such chemicals into the body, their differing stabilities, and elimination are difficult to model, it is much more straightforward to assay for these compounds in the body rather than in the environment. The primary innovations in our approach are that a novel optical micro-to-nano-fluidic device will be used along with unique aptamers and Raman reporter molecules to simultaneously measure several classes of compounds in a multiplexed fashion. The opto-fluidic device is an extremely sensitive surface enhanced Raman spectroscopy (SERS) nanochannel cartridge that was invented at Texas A&M University by professors Jun Kameoka and Gerard Coté. The aptamer functionalization chemistry as well as the overall integrated product will be developed at BioTex, Inc. with aptamers discovered at sister company, Base Pair Biotechnologies. The device being developed provides signal enhancements equal to or exceeding 1014 at the entrance to the nanochannel, enabling rapid quantitation of femtomolar or smaller levels of targets in fluids. As demonstrated in Phase I, the individual components of the system have been thoroughly tested, and the Phase II study will therefore focus on the engineering integration and demonstration of overall system performance. The resonant-SERS reporting and nanofluidic concentrator employed have the potential to provide orders-of-magnitude improved sensitivity over standard fluorescence and without the complicated multistep tasks of ELISA or full analytical chemistry analysis such as chromatography and mass spectrometry. Using aptamers to virtually any environmental compound of concern, the fully-developed platform will be able to provide quantitative "point-of-care" or field results in a matter of minutes.

描述（由申请人提供）：NIH-SBIR 第二阶段工作的目标是开发一种新的即时护理 (POC) 检测平台和方法，用于评估人体暴露于血液中有害环境化合物的机制。这些化学物质进入体内的运输过程、它们不同的稳定性和消除都很难建模，在体内而不是在环境中检测这些化合物要简单得多。我们方法的主要创新在于一种新颖的光学方法。微纳米流体装置将与独特的适体和拉曼报告分子一起使用，以多重方式同时测量几类化合物。该光流体装置是一种极其灵敏的表面增强拉曼光谱（SERS）纳米通道盒。由德克萨斯 A&M 大学的 Jun Kameoka 和 Gerard Coté 教授发明。适体功能化化学以及整体集成产品将在 BioTex, Inc. 与适体一起开发。姐妹公司 Base Pair Biotechnologies 发现，正在开发的设备在纳米通道入口处提供等于或超过 1014 的信号增强，从而能够快速定量流体中的飞摩尔级或更小水平的目标，如第一阶段所示，各个组件。该系统的性能已经过彻底测试，因此第二阶段研究将重点关注整体系统性能的工程集成和演示，所使用的共振SERS报告和纳米流体聚光器有潜力提供。与标准荧光相比，灵敏度提高了几个数量级，并且不需要 ELISA 或完整的分析化学分析（例如色谱法和质谱法）的复杂多步骤任务，该完全开发的平台将能够提供几乎任何关注的环境化合物。几分钟内即可获得定量的“现场护理”或现场结果。