Portable nanofluidic aptamer-SERS instrument for measurement of chemical exposure

用于测量化学暴露的便携式纳米流体适体-SERS 仪器

基本信息

批准号：
8431721
负责人：
George W Jackson
金额：
$ 24.89万
依托单位：
BIOTEX, INC.
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-19 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8431721
关键词：
Address Ambulances Analytical Chemistry Biochemical Biological Assay Biological Monitoring Blood Blood Circulation Blood Tests Budgets Carcinogens Chemical Exposure Chemical Structure Chemicals Chemistry Chromatography Complex Complex Mixtures DNA Detection Devices Environment Environmental Exposure Enzyme-Linked Immunosorbent Assay Exposure to Fluorescence Free Will Goals Gold Hand Housing Individual Laboratories Lasers Ligands Light Liquid substance Mass Spectrum Analysis Measurement Measures Metals Methodology Microfluidics Modeling Monitor Nanotechnology Optics Phase Physicians Polychlorinated Biphenyls Publications Raman Spectrum Analysis Reporter Reporting Research Research Design Research Personnel Side Signal Transduction Small Business Innovation Research Grant Source Spectrum Analysis Spottings Surface System Systems Analysis Techniques Technology Testing Texas United States National Institutes of Health Universities Whole Blood aptamer base bisphenol A blood filtration clinical application cost effective design detector environmental agent exposed human population improved innovation instrument instrumentation mass spectrometer molecular recognition nanochannel nanofluidic nanoparticle novel phthalates point of care process optimization professor programs stressor

项目摘要

DESCRIPTION (provided by applicant): Project Summary The goal of this NIH-SBIR Phase I proposal is to develop a new point-of-care (POC) detection platform and methodology for assessment of human exposure to hazardous environmental compounds in the bloodstream. Because the mechanisms of transport of such chemicals into the body, their differing stabilities, and elimination are difficult to model, if possible, it is much more straightforward to assay for these compounds in the body rather than in the environment. The primary innovations in our approach are that a novel optical micro- to nano-fluidic device will be used along with unique aptamers and Raman reporter molecules to simultaneously measure several classes of compounds in a multiplexed fashion. The opto-fluidic device is an extremely sensitive surface enhanced Raman spectroscopy (SERS) nanochannel cartridge that was invented at Texas A&M University by professors Jun Kameoka and Gerard Cot¿. The aptamer functionalization chemistry as well as the overall integrated product will be developed at BioTex, Inc. The device being developed provides signal enhancements equal to or exceeding 1014 at the entrance to the nanochannel, enabling rapid quantification of femtomolar or smaller levels of targets in fluids. This SERS-based nanofluidic platform has been demonstrated through several publications and so the key question that need to be addressed by the company are can the aptamer chemistry perform as intended in progressively complex media including whole blood with this platform and can the platform be optimized for this application. Thus, the specific aims of this research are to develop the aptamer assay for specifically detecting model polychlorinated biphenyls (PCBs), bisphenol-A (BPA), and model phthalates. The resonant-SERS reporting and nanofluidic concentrator employed have the potential to provide orders-of-magnitude improved sensitivity over standard fluorescence and without the complicated multistep tasks of ELISA or full analytical chemistry analysis such as chromatography and mass spectrometry. PUBLIC HEALTH RELEVANCE: Project Narrative The goal of this research is to develop a new point-of-care (POC) detection platform and methodology for assessment of biological exposure to harmful chemical compounds. With the potential to greatly improve the sensitivity and ease of such measurements, the end-product of this research is expected to have considerable impact allowing toxicologists, environmental health professionals, and clinicians the ability to correlate the effects of toxic chemicals to disease states.

描述（由申请人提供）：项目摘要 NIH-SBIR 第一阶段提案的目标是开发一种新的即时护理 (POC) 检测平台和方法，用于评估人体血液中有害环境化合物的暴露情况。这些化学物质进入体内的机制、它们不同的稳定性和消除很难建模，如果可能的话，在体内而不是在环境中检测这些化合物要简单得多。我们方法的主要创新是。一种新颖的光学微米到纳米流体装置将与独特的适体和拉曼报告分子一起使用，以多重方式同时测量几类化合物。该光流体装置是一种极其灵敏的表面增强拉曼光谱（SERS）纳米通道盒。由 Jun Kameoka 和 Gerard Cot 教授在德克萨斯 A&M 大学发明适体功能化化学以及整体集成产品将由 BioTex, Inc. 开发。正在开发的设备可在纳米通道入口处提供等于或超过 1014 的信号增强，从而能够快速定量流体中飞摩尔或更小水平的目标这种基于 SERS 的纳米流体平台已通过多篇出版物得到证实，因此该公司需要解决的关键问题是适体化学能否在逐渐复杂的介质（包括整个介质）中发挥预期作用。因此，本研究的具体目标是开发用于特异性检测模型多氯联苯 (PCB)、双酚 A (BPA) 和模型共振邻苯二甲酸盐的适体检测方法。 - 采用的 SERS 报告和纳米流体浓缩器有可能比标准荧光提供数量级的灵敏度提高，并且无需 ELISA 或完整分析化学分析的复杂多步骤任务例如色谱法和质谱法。公共健康相关性：项目叙述本研究的目标是开发一种新的即时护理 (POC) 检测平台和方法，用于评估有害化合物的生物暴露，有可能大大提高此类测量的灵敏度和简易性。，这项研究的最终产品预计将产生相当大的影响，使毒理学家、环境卫生专业人员和公众能够将有毒化学品的影响与疾病状态联系起来。