Development of a turnkey system for assessing forelimb function in rats and mice.

开发用于评估大鼠和小鼠前肢功能的交钥匙系统。

基本信息

批准号：
8715164
负责人：
Andrew Michael Sloan
金额：
$ 13.02万
依托单位：
VULINTUS, LLC
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-01 至 2014-09-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8715164
关键词：
Affect American Animal Behavior Animal Experimentation Animal Model Animals Behavioral Assay Behavioral Research Clinic Clinical Communities Data Detection Development Diabetes Mellitus Digit structure Disease Dose Engineering Ensure Feedback Female Forelimb Foundations Generations Housing Hypertension Industry Injection of therapeutic agent Ischemic Stroke Isometric Exercise Laboratories Laboratory Research Lead Life Marketing Measures Mechanics Medical Device Metals Methods Modeling Molds Motor Movement Mus National Institute of Neurological Disorders and Stroke Neurologic Operant Conditioning Outcome Outcome Assessment Parkinson Disease Performance Pharmacologic Substance Phase Physiological Pre-Clinical Model Production Pronation Rattus Recommendation Reporting Research Research Contracts Research Personnel Resources Rodent Running Sample Size Sampling Site Small Business Innovation Research Grant Speed Spinal cord injury Stroke Suggestion Supination System Technology Test Result Testing Training Translating Translations United States National Institutes of Health Ursidae Family Validation World Health Organization aged clinically relevant cost disability experience hypercholesterolemia improved kinematics male motor deficit motor disorder nervous system disorder novel platform-independent portability pre-clinical therapy preclinical study programs public health relevance relating to nervous system research study response scale up stroke therapy success therapy development

项目摘要

DESCRIPTION (provided by applicant): Millions of Americans are affected by neurological disorders such as stroke and spinal cord injury. These disorders often result in impaired motor function and lead to significant disability amongst those affected. Despite major efforts to develop therapies to alleviate the burden of these disorders, virtually all promising preclinical therapies fail to successfully translate to the clinic. Reports from the Stroke Therapy Academic Industry Roundtable and the NINDS suggest that preclinical studies that eliminate outcome bias and ensure appropriate power would have a substantial impact on improving the predictive power of these studies, and consequently improve the development and successful translation of therapies. Current state-of-the-art preclinical models that evaluate motor function are exceedingly labor intensive, bear the potential for experimenter bias, and cannot reasonably be scaled up. Here, we propose to fully develop our novel MotoTrak system, the first high-throughput, automated rodent operant conditioning system able to isolate, quantify, and track skilled forelimb performance in rats and mice. The system quantifies a wide range of clinically relevant parameters, including forelimb force generation, digit extension/flexion, supination/pronation, and kinematic movement speed. A single minimally trained technician can run a dozen animals simultaneously and obtain approximately 1000 trial samples per rat per day. The amount and accuracy of the data collected represents a substantial improvement over current available motor assessment systems. Vulintus proposes to develop MotoTrak from a laboratory-grade system to a commercially viable product and market the system to academic research laboratories, pharmaceutical companies, medical device companies, and contract research organizations across the world. With MotoTrak, Vulintus has the potential to transform the field of motor behavioral research and subsequently enhance the development of therapies to treat neurological disorders.

描述（由申请人提供）：数百万美国人受到中风和脊髓损伤等神经系统疾病的影响。这些疾病通常会导致运动功能受损，并导致受影响者出现严重残疾。尽管努力开发疗法来减轻这些疾病的负担，但几乎所有有前途的临床前疗法都未能成功转化为临床。中风治疗学术行业圆桌会议和 NINDS 的报告表明，消除结果偏差并确保适当功效的临床前研究将对提高这些研究的预测能力产生重大影响，从而改善治疗的开发和成功转化。目前评估运动功能的最先进的临床前模型极其耗费人力，可能存在实验者偏差，并且无法合理扩大规模。在这里，我们建议全面开发我们的新型 MotoTrak 系统，这是第一个高通量、自动化啮齿动物操作调节系统，能够分离、量化和跟踪大鼠和小鼠的熟练前肢表现。该系统量化了广泛的临床相关参数，包括前肢力的产生、手指伸展/屈曲、旋后/旋前和运动速度。一名经过最低限度培训的技术人员可以同时运行十几只动物，每天为每只大鼠获取大约 1000 个试验样本。所收集数据的数量和准确性比当前可用的运动评估系统有了实质性的改进。 Vulintus 提议将 MotoTrak 从实验室级系统开发为商业上可行的产品，并将该系统销售给世界各地的学术研究实验室、制药公司、医疗器械公司和合同研究组织。凭借 MotoTrak，Vulintus 有潜力改变运动行为研究领域，并随后促进神经系统疾病疗法的开发。