Modeling Mammalian Genomes

哺乳动物基因组建模

• 批准号: 8135058
• 负责人: C. Anthony Blau
• 金额: $ 60.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-30 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8135058
• 关键词: Adopted; Alleles; Architecture; CCCTC-binding factor; Cells; Cellular biology; Centromere; Chromatin; Chromosomes; Code; Complex; DNA replication origin; Data; Data Set; Development; Diploidy; EP300 gene; Enhancers; Epigenetic Process; Frequencies; Functional RNA; Fungal Genome; Genes; Genetic Transcription; Genome; Genomics; Genotype; Haploidy; Hybrid Cells; Hybrids; Hypoxanthine Phosphoribosyltransferase; Kidney; Light; Maps; Methods; Modeling; Molecular Conformation; Mus; Nature; Phenotype; Protein Binding; RNA; Research Personnel; Rodent; Role; Saccharomyces cerevisiae; Scaffolding Protein; Single Nucleotide Polymorphism; Structure; Structure-Activity Relationship; Surveys; Techniques; Technology; Transfer RNA; United States National Institutes of Health; X Chromosome; base; cell type; cohesin; genetic element; genome-wide; histone modification; mammalian genome; promoter; spatial relationship; telomere; three-dimensional modeling

DESCRIPTION (provided by applicant): Recently, genomes have been recognized to adopt preferred physical architectures that are evolutionarily conserved and cell type specific. We developed a method for characterizing long-range inter- and intra- chromosomal interactions on a genome-wide scale. Our method is based on chromatin conformation capture (3C) but unlike 3C (or other derivative technologies), makes no a priori assumptions regarding interacting genetic elements. Due to the large size of mammalian genomes we initially validated our approach in haploid Saccharomyces cerevisiae. In this application we propose to use this method to understand the relationship between genome architecture and allele specific expression in mammalian development.

描述（由申请人提供）：最近，基因组已被认为采用进化上保守且细胞类型特异性的优选物理结构。我们开发了一种在全基因组范围内表征长程染色体间和染色体内相互作用的方法。我们的方法基于染色质构象捕获 (3C)，但与 3C（或其他衍生技术）不同，我们的方法没有对相互作用的遗传元件做出先验假设。由于哺乳动物基因组较大，我们最初在单倍体酿酒酵母中验证了我们的方法。在本申请中，我们建议使用这种方法来了解哺乳动物发育中基因组结构与等位基因特异性表达之间的关系。