Self Renewal and Differentiation of Embryonic Stem Cells

胚胎干细胞的自我更新和分化

• 批准号: 7303498
• 负责人: C. Anthony Blau
• 金额: $ 192.83万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7303498
• 关键词: Self; Renewal; Differentiation; Embryonic; Stem

DESCRIPTION (provided by applicant): This application, entitled "Self Renewal and Differentiation of Human Embryonic Stem Cells," is comprised of our Human ES Cell Core Laboratory and four projects. We propose to dramatically expand the work begun during our current funding as a University of Washington/Fred Hutchinson Cancer Research Center (UW/FHCRC) "Exploratory Center for Human Embryonic Stem Ceil Research." Our Human ES Cell (hESC) Core Lab, directed by Carol Ware, has acquired and characterized 14 of the 21 NIH approved human ESC lines, and has assisted 18 laboratories in work related to hESCs. The Core will acquire the remaining NIH approved hESC lines, will characterize microRNA expression profiles of all lines, assist all of the component projects, and will also continue to assist in making hESCs accessible to a local community of more than 80 labs with interests relevant to hESCs. Project 1, led by Drs. Blau and Ware, will examine an alternative pathway for self-renewal that is activated by the short chain fatty acid, butyrate. Project 2, led by Randall Moon, will examine canonical and non-canonical Wnt signaling pathways in hESCs. Project 3, headed by Charles Murry, examines hESCs during differentiation directed toward a cardiomyocyte fate while Project 4, led by Tom Reh, evaluates directed differentiation toward a neuroretinal fate. Logical and extensive interactions between each of the projects, and extensive interactions between the hESC Core Lab, each of the projects, and other labs at UW/FHCRC create a Program vastly stronger than the sum of its parts.

描述（由申请人提供）：该申请题为“人类胚胎干细胞的自我更新和分化”，由我们的人类胚胎干细胞核心实验室和四个项目组成。我们建议大幅扩展我们目前资助期间开始的工作，作为华盛顿大学/弗雷德·哈钦森癌症研究中心 (UW/FHCRC)“人类胚胎干细胞研究探索中心”。我们的人类 ES 细胞 (hESC) 核心实验室由 Carol Ware 领导，已获得并鉴定了 21 个 NIH 批准的人类 ESC 系中的 14 个，并协助 18 个实验室开展与 hESC 相关的工作。核心将收购剩余的 NIH 批准的 hESC 系，将表征所有系的 microRNA 表达谱，协助所有组成项目，并将继续协助使 80 多个与相关利益相关的实验室组成的当地社区能够获得 hESC。人类胚胎干细胞。项目 1，由博士领导。 Blau 和 Ware 将研究一种由短链脂肪酸丁酸激活的自我更新替代途径。项目 2 由 Randall Moon 领导，将检查 hESC 中的规范和非规范 Wnt 信号通路。由 Charles Murry 领导的项目 3 检查 hESC 向心肌细胞命运的分化过程，而由 Tom Reh 领导的项目 4 评估向神经视网膜命运的定向分化。每个项目之间逻辑性和广泛的互动，以及 hESC 核心实验室、每个项目以及 UW/FHCRC 的其他实验室之间的广泛互动，创建了一个比各个部分之和强得多的计划。