Macromolecular Crystallography at the National Synchrotron Light Source

国家同步加速器光源下的大分子晶体学

基本信息

批准号：
7928626
负责人：
ROBERT M SWEET
金额：
$ 201.61万
依托单位：
BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-10-02 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7928626
关键词：
2-Nitropropane dioxygenase Abate Age Alkylating Agents Aprotinin Area Arts Asthma Automation Biological Preservation Biological Sciences Biology Blood capillaries Carbon Chemicals Choline Citric Acid Cycle Client Collaborations Collimator Communities Complex Computer software Core Facility Crystallization Crystallography Culicidae Cyanobacterium Cyanothece Cyclic AMP Receptor Protein Cytomegalovirus DNA Data Data Collection Data Set Databases Death Domain Deposition Development Devices Diagnostic Effectiveness Environment Equipment Equus caballus European Flavoproteins Floor Fluorescence Fluorescence Spectrophotometry Fluorescence Spectroscopy France Freezing Future Gases Generations Goals Grant Growth HIV Heart Helium Heme Human Hybrids Hypoxia Image Imagery Indium Institutes International Laboratories Lasers Lead Ligand Binding Light Link Logic Mails Measurement Metabolism Methods Microfluidics Microspectrophotometry Mixed Function Oxygenases Modeling Molecular Biology Motion Mycobacterium tuberculosis Myoglobin Names Nitric Oxide Nitrites Nitrogen Nitrogen Fixation Occupations Online Systems Operative Surgical Procedures Optics Oxidases Oxidoreductase Pathway interactions Performance Phase Population Preparation Price Process Program Development Proteins Publications RNA Radiation Raman Spectrum Analysis Reader Recombinant Proteins Recruitment Activity Reporting Research Resolution Resources Robotics Roentgen Rays Sampling Scientist Screening procedure Services Shapes Signal Transduction Silicon Site Source Specific qualifier value Specimen Specimen Handling Spectrum Analysis Speed Spliceosomes Staging System Structure Switzerland Synchrotrons System Technology Thermodynamics Time Training Transaminases Tryptophan Visible Radiation Work absorption base beamline capillary copper oxidase cryogenics design detector digital esterase experience firewall flexibility human disease improved infrared spectroscopy inhibitor/antagonist instrument mRNA Precursor member mesotrypsin multicatalytic endopeptidase complex nitric oxide reductase novel oxidation polyamine oxidase pressure programs research and development research study response structural biology transcription factor

项目摘要

DESCRIPTION (provided by applicant): The Macromolecular Crystallography Research Resource (PXRR) at the National Synchrotron Light Source (NSLS) operates six beamlines that are employed for research by about 160 outside research groups and half a dozen Brookhaven Lab groups. These scientists produce over 200 research publications in a typical year, and over 300 Protein Data Bank depositions in the past year. The PXRR have pioneered an active Mail-in program that involves ongoing collaborations with about 15 outside research groups and short collaborations or service projects with many more. The Mail-in program has interacted with 90 groups in the past several years; each year for the past four years there have been about 25 PDB depositions and 15 publications from the Mail-in program. The PXRR performs research and development on apparatus, methods, and software to improve the technology for synchrotron-based macromolecular crystallography. Special emphases during the coming cycle will be on the development of detectors that will match the power and collimation of modern x-ray sources, the use of micron-sized crystals, the development of robotic cryogenic specimen handling, and the simultaneous measurement of UV, Visible, or Raman IR spectra with x-ray diffraction. The PXRR staff will execute numerous small research programs, among other things to improve visualization of tiny specimens and improve cryogenic preservation of specimens. A long-term goal for the PXRR is to create a resource for macromolecular crystallography at NSLS-II, the new synchrotron light source that is being created at Brookhaven Lab during the next five to seven years. The objective will be to have several macromolecular crystallography beamlines in operation when NSLS-II begins operation. This project will involve direct cooperation with NSLS-II staff to develop a complete plan for such facilities, and then to begin to achieve that plan.

描述（由申请人提供）：国家同步加速器光源 (NSLS) 的大分子晶体学研究资源 (PXRR) 运行 6 条光束线，约 160 个外部研究小组和 6 个布鲁克海文实验室小组使用这些光束线进行研究。这些科学家通常每年发表 200 多篇研究出版物，并在过去一年发表 300 多篇蛋白质数据库沉积物。 PXRR 开创了一项积极的邮寄计划，该计划涉及与大约 15 个外部研究小组的持续合作以及与更多小组的短期合作或服务项目。过去几年，邮寄计划已与 90 个团体进行了互动；过去四年中，每年都会有大约 25 份 PDB 证词和 15 份来自 Mail-in 计划的出版物。 PXRR 对设备、方法和软件进行研究和开发，以改进基于同步加速器的高分子晶体学技术。下一个周期的特别重点将是开发与现代 X 射线源的功率和准直相匹配的探测器、微米级晶体的使用、机器人低温样本处理的开发以及同时测量紫外线、可见光或拉曼红外光谱与 X 射线衍射。 PXRR 工作人员将执行许多小型研究项目，其中包括改善微小标本的可视化和改善标本的低温保存。 PXRR 的长期目标是为 NSLS-II 创建大分子晶体学资源，NSLS-II 是布鲁克海文实验室在未来五到七年内创建的新型同步加速器光源。目标是当 NSLS-II 开始运行时，有多条大分子晶体学光束线投入运行。该项目将涉及与 NSLS-II 工作人员的直接合作，为此类设施制定完整的计划，然后开始实现该计划。