Imaging Core

成像核心

• 批准号: 10618813
• 负责人: Bruce J. Tromberg
• 金额: $ 17.03万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10618813
• 关键词: Acceleration; Animal Experiments; Animal Model; Animals; Biological; Biology; Biophotonics; Blood Vessels; Cells; Center Core Grants; Clinic; Clinical Trials; Collaborations; Collagen; Communities; Computer Models; Computing Methodologies; Data Set; Dedications; Development; Disease; Elastin; Elements; Extracellular Matrix; Feedback; Fluorescence; Functional disorder; Funding; Genetic Engineering; Genomics; Goals; Housing; Human; Image; Imaging technology; In Vitro; Keratin; Knowledge; Label; Laboratories; Laser Speckle Imaging; Lasers; Light; Lipids; Medical; Medicine; Melanins; Methodology; Methods; Microscopic; Modality; Modeling; Molecular; Molecular Probes; NADH; Optical Coherence Tomography; Optics; Patient-Focused Outcomes; Performance; Phenotype; Process; Reporter; Research; Research Personnel; Resources; Signal Transduction; Skin; Skin Tissue; Spatial Frequency Domain Imaging; Systems Biology; Technology; Testing; Tissues; Training; Translating; United States National Institutes of Health; Visualization; collaborative environment; data integration; experience; experimental study; fluorescence imaging; fluorescence lifetime imaging; genomic data; imaging approach; imaging capabilities; imaging modality; imaging platform; improved outcome; in vivo; in vivo optical imaging; insight; mathematical model; member; microscopic imaging; molecular imaging; multimodality; multiphoton microscopy; non-invasive optical imaging; novel; novel strategies; optical imaging; photonics; pre-clinical; skin disorder; tissue fixing; tool; virtual

PROJECT SUMMARY/ABSTRACT The broad objective of the Imaging Core is to use cutting edge optical imaging technologies to non-invasively visualize the macroscopic, microscopic, and molecular processes that underlie skin biology and disease. The Core builds on technologies and expertise in the Beckman Laser Institute and Medical Clinic (BLI) and the Laboratory for Fluorescence Dynamics (LFD), longstanding UC Irvine centers that have pioneered the development and application of optics and photonics technologies for biology and medicine. BLI and LFD will create a new skin imaging suite housing specific, state-of-the-art, in vivo optical imaging platforms to P30 skin biology investigators, including: Multiphoton Microscopy, MPM; Fluorescence Lifetime Imaging Microscopy, FLIM; Coherent Raman Scattering, CRS; Spatial Frequency Domain Imaging, SFDI; Laser Speckle Imaging, LSI; and Optical Coherence Tomography (OCT); as well as computational methods and models for understanding light propagation in skin (i.e. skin optics). These technologies are used to visualize fluorescent molecular probes and reporters as well as provide label-free contrast from intrinsic tissue signals in cells, ECM, and vasculature. By employing these multi-modal, multi-scale technologies, the imaging core will facilitate studies that can lead to powerful new skin biology insights that can be translated to humans. This is accomplished by integrating imaging datasets with the Genomics and Systems biology cores to develop models that can then be tested in biological experiments, including in animals. In addition to providing cutting edge technologies for research, a key component of the Imaging Core will be to provide resources for training, dissemination, and collaboration that drive and support interdisciplinary activities. Our goal is to bring together technology developers, modeling experts, biologists, and clinicians both within UCI and the skin research community at large to build teams that can share knowledge and apply new tools to solve longstanding problems. This will be accomplished by creating a combination of hands-on training, didactic training, and dissemination activities that provide skin biology researchers with the knowledge, resources, and support needed to integrate state-of the-art optical imaging into skin biology research. Finally, our technologies will continue to be optimized, with feedback from the community, to better understand skin biology and pathophysiology, with the ultimate goal of developing new approaches that improve outcomes for patients suffering from cutaneous disease.

项目概要/摘要 成像核心的广泛目标是利用尖端光学成像技术以非侵入性方式 可视化皮肤生物学和疾病的宏观、微观和分子过程。这 核心建立在贝克曼激光研究所和医疗诊所 (BLI) 以及 荧光动力学实验室 (LFD)，是加州大学欧文分校的长期中心，开创了荧光动力学 生物和医学光学和光子技术的开发和应用。 BLI 和 LFD 将 创建一个新的皮肤成像套件，其中包含针对 P30 皮肤的特定、最先进的体内光学成像平台 生物学研究人员，包括：多光子显微镜、MPM；荧光寿命成像显微镜， FLIM；相干拉曼散射，CRS；空间频域成像，SFDI；激光散斑成像， 大规模集成电路；和光学相干断层扫描（OCT）；以及计算方法和模型 了解皮肤中的光传播（即皮肤光学）。这些技术用于可视化荧光 分子探针和报告基因，并提供与细胞、ECM、 和脉管系统。通过采用这些多模态、多尺度技术，成像核心将促进 这些研究可以带来强大的新皮肤生物学见解，并可以转化为人类。这是 通过将成像数据集与基因组学和系统生物学核心集成来开发 然后可以在生物实验（包括动物实验）中测试模型。除了提供切割 研究的边缘技术，成像核心的一个关键组成部分将是提供培训资源， 推动和支持跨学科活动的传播和协作。我们的目标是汇聚 UCI 和皮肤研究领域的技术开发人员、建模专家、生物学家和临床医生 整个社区建立可以共享知识并应用新工具来解决长期问题的团队 问题。这将通过实践培训、教学培训和 为皮肤生物学研究人员提供知识、资源和支持的传播活动 需要将最先进的光学成像整合到皮肤生物学研究中。最后，我们的技术将 根据社区的反馈继续优化，以更好地了解皮肤生物学和 病理生理学，最终目标是开发改善患者预后的新方法 患有皮肤病。