Molecular Biology Core

分子生物学核心

• 批准号: 10618376
• 负责人: Jon Scott Blevins
• 金额: $ 22.8万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-05 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10618376
• 关键词: Address; Antibody Formation; Applications Grants; Arkansas; Award; Centers of Research Excellence; Cloning; Core Facility; DNA; DNA Sequencing Facility; DNA biosynthesis; DNA sequencing; Data; Dedications; Doctor of Philosophy; Ensure; Equation; Equipment; Fee-for-Service Plans; Fees; Flow Cytometry; Funding; Gel; Genes; Genomic Library; Genomics; Goals; Growth; Human Resources; Immunology; Inflammatory; Inflammatory Response; Institution; Investments; Laboratory Personnel; Maintenance; Manuscripts; Medical; Medicine; Methods; Microbial Genome Sequencing; Microbiology; Modernization; Molecular Biology; Opportunistic Infections; Pathogenesis; Phase; Preparation; Proteins; RNA; Recovery; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Science; Scientist; Service delivery model; Services; Side; Supervision; Technology; Time; Training; Universities; Validation; Western Blotting; Work; base; cellular imaging; college; cost; equipment acquisition; genome-wide; instrument; instrumentation; microbial; microscopic imaging; next generation; next generation sequencing; operation; pathogen; programs; protein expression; protein purification; quantitative imaging; response; success; tool; user-friendly; voucher

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. One of these was a DNA Sequencing Core. However, the capability of this core was limited to single-gene sequencing. While this was an important and necessary service, it was not sufficient, particularly in an era of genomic and next-generation sequencing technology. Thus, the CMPHIR also invested heavily in augmenting the resources available in the existing DNA Sequencing Core. We accomplished this task, and, in Phase III, we propose to integrate these services under the common umbrella of a Molecular Biology Core (Core B) capable of providing CMPHIR and other UAMS investigators with the resources they need, particularly with respect to the pathogen side of the host–pathogen equation. The activities of Core B include enhancing the capability of obtaining high-quality RNA, DNA, and protein samples for analysis; high-throughput single-gene sequencing using conventional Sanger methods; next-generation sequencing of microbial genomes and genomic libraries; protein expression and purification; and antibody production. The proposed Core B will be supported through a combination of CMPHIR funding and continued institutional support. Daily operations will be supervised by Dr. Jon S. Blevins, Director, who will work with 2 full-time technicians. One of these technicians will have primary responsibility for the DNA component of Core B, while the other will have primary responsibility for the protein component. To ensure consistency and promote self-sustainability, Core B instruments will be operated by these technicians on behalf of CMPHIR and UAMS investigators on a fee-for-service basis. This will allow us to recover a significant proportion of the costs and thereby ensure the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.

项目概要/摘要 在第一阶段和第二阶段，微生物发病机制和宿主炎症反应中心 (CMPHIR) 建立并维持一个研究和技术进步核心，以填补实验空白 UAMS 医学院维护的机构核心中可用的资源之一就是 DNA。 然而，该核心的功能仅限于单基因测序。 重要且必要的服务，但这还不够，特别是在基因组和下一代时代 因此，CMPHIR 还投入了大量资金来增加可用资源。 我们完成了这项任务，并且在第三阶段，我们建议整合这些。 分子生物学核心（核心 B）的共同保护伞下的服务能够提供 CMPHIR 和 其他 UAMS 调查人员拥有他们所需的资源，特别是在病原体方面 Core B 的活性包括增强获得高质量 RNA 的能力， 使用传统 Sanger 进行高通量单基因测序分析； 微生物基因组和基因组蛋白表达的新一代测序； 拟议的核心 B 将通过 CMPHIR 的组合得到支持。 资金和持续的机构支持将由董事 Jon S. Blevins 博士监督。 他将与 2 名全职技术人员一起工作，其中一名技术人员将主要负责 DNA。 核心B的组成部分，而另一个将主要负责蛋白质组成部分以确保。 一致性并促进自我可持续性，Core B仪器将由这些技术人员代表操作 CMPHIR 和 UAMS 调查员按服务收费，这将使我们能够收回大量资金。 成本比例，从而确保该核心设备的升级和维护能力 当我们进入第三阶段及以后阶段时需要。