CRF IN THE AMYGDALA--ELECTROPHYSIOLOGY AND PHARMACOLOGY

杏仁核中的 CRF——电生理学和药理学

• 批准号: 3416064
• 负责人: PATRICIA SHINNICK-GALLAGHER
• 金额: $ 12.03万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3416064
• 关键词: Mammalia; cell type; cerebellar cortex; corticotropin releasing factor; electrophysiology; membrane potentials; neural transmission; neuroanatomy; neurons; neuropharmacology; second messengers; stainings; synapses

DESCRIPTION: (Investigator's Abstract) This study will test the hypothesis that the action of CRF on proposed autoreceptors in the central nucleus of the amygdala is different than on postsynaptic CRF receptors receiving CRF projections. The following specific aims will be addressed. 1) analyze CRF effects on electrophysiological properties of neurons in the two nuclei; 2) determine the mechanisms of action on specific membrane currents underlying the action of CRF; 3) correlate cell types with the differential response to CRF in central and basolateral amygdala using intracellular staining with Lucifer Yellow; 4) examine possible second messenger systems involved in the actions of CRF in the different nuclei; and 5) determine possible presynaptic modulatory actions of CRF on synaptic transmission. Intracellular recordings from a submerged brain slice preparation will be used in current and single electrode voltage clamp modes to address these aims.

描述：（研究者摘要）本研究将检验假设 CRF 对中央核中提议的自身受体的作用 杏仁核与接受 CRF 的突触后 CRF 受体不同 预测。 将解决以下具体目标。 1）分析 CRF对两者神经元电生理特性的影响 原子核； 2) 确定特定膜电流的作用机制 CRF 作用的基础； 3）将细胞类型与差异相关联 使用细胞内对中央和基底外侧杏仁核的 CRF 的反应 用荧光黄染色； 4）检查可能的第二信使系统 参与CRF在不同细胞核中的作用； 5) 确定 CRF 对突触传递可能的突触前调节作用。 来自水下脑切片制剂的细胞内记录将被 用于电流和单电极电压钳模式来解决这些问题 目标。