Effects of environmental contaminants on anxiety-like and fear behaviors, and gut-microbiota in rodents

环境污染物对啮齿动物焦虑样和恐惧行为以及肠道微生物群的影响

基本信息

批准号：
10908899
负责人：
FILIPA GODOY-VITORINO
金额：
$ 6.91万
依托单位：
UNIVERSITY OF PUERTO RICO MED SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10908899
关键词：
Adult Affect Agriculture Amygdaloid structure Animals Anxiety Bacteria Behavior Behavioral Biological Brain Brain region Breathing Chemicals Clinical Consumption Darkness Development Dill Disease Dose Emotional disorder Environmental Pollutants Environmental Pollution Enzymes Event Exposure to FOS gene Female Food Food Preservatives Freezing Fright Functional disorder Future Goals Gonadal Hormones Grant Health Herbicides Histology Human Knowledge Light Link Mammals Measures Memory impairment Mental disorders Metabolic Minority Groups Mission Movement National Institute of Environmental Health Sciences National Institute of Mental Health National Institute on Minority Health and Health Disparities Neurodevelopmental Disorder Neurologic Neurons Neurotransmitters Pathogenesis Pathway interactions Plants Predisposition Prefrontal Cortex Rattus Reporting Research Domain Criteria Resistance Risk Rodent Role Route Sampling Serotonin Sex Differences Structure Structure of terminal stria nuclei of preoptic region Testing Time Toxic Environmental Substances United States Environmental Protection Agency United States Food and Drug Administration United States National Institutes of Health Water anxiety-like behavior arm behavior change behavior test behavioral study brain circuitry comparison control conditioned fear contaminated drinking water contaminated water drinking dysbiosis emotion dysregulation exposed human population fear memory fecal microbiota glyphosate gut microbes gut microbiota industrial production male microbial based therapy microorganism neural pathogen preclinical study response sex shikimate symbiont

项目摘要

Project Summary Our goal is to understand the effects of environmental contaminants, namely glyphosate, on anxiety-like and fear-related behaviors, and determine possible links between gut microbial imbalances and behavioral deficits. The increased risk of human exposure to chemicals from food preservatives, agriculture and industrial production suggests a link between exposure to environmental toxins, such as glyphosate, and the development of neurological and emotional disorders. Glyphosate, the active ingredient in glyphosate-based herbicides, was initially considered safe for mammals since it exerts its effect by inhibiting a metabolic route not present in mammals. However, this enzyme is found in microorganism, making the gut microbiota likely susceptible to damage by glyphosate. Given that the gut microbiota benefits the host, by acting as a defense against pathogens, producing neurotransmitters such as serotonin, and regulating normal brain function, disturbances of the gut microbiota may contribute to neural pathogenesis by generating a cascade of events which lead to emotional dysregulation. Thus, glyphosate can impact the health of animals by acting against their gut microbes. The influence of glyphosate on anxiety-like and fear-related behaviors is unclear. To evaluate the potential relationship between the influence of glyphosate on anxiety, fear, and gut microbiota, a link must be examined. Understanding how glyphosate impacts rat gut symbionts, their behavior and brain pathophysiology will help elucidate a possible role of this chemical to anxiety and fear. We will give either glyphosate-contaminated drinking water or filtered water, to adult rats, and perform behavioral studies for anxiety-like and fear-related behaviors. We hypothesize that prolonged drinking of glyphosate-contaminated water will lead to increased anxiety-like and fear-related behaviors. In addition, by evaluating for changes in the composition of gut microbiota, we will identify microbial imbalances that may correlate with the development of emotional disorders.

项目概要我们的目标是了解环境污染物（即草甘膦）对焦虑样和恐惧相关的行为，并确定肠道微生物之间可能的联系失衡和行为缺陷。人类接触化学物质的风险增加食品防腐剂、农业和工业生产表明接触食品防腐剂之间存在联系环境毒素，如草甘膦，与神经和情绪的发展失调。草甘膦是草甘膦除草剂的活性成分，最初是被认为对哺乳动物是安全的，因为它通过抑制不存在的代谢途径来发挥作用在哺乳动物中。然而，这种酶存在于微生物中，使得肠道微生物群很可能易受草甘膦损害。鉴于肠道微生物群通过作用对宿主有益作为对病原体的防御，产生神经递质，如血清素，并调节正常的大脑功能，肠道微生物群的紊乱可能导致神经发病机制通过产生一系列导致情绪失调的事件。因此，草甘膦可以通过对抗肠道微生物来影响动物的健康。草甘膦的影响焦虑样和恐惧相关行为的影响尚不清楚。评估潜在关系草甘膦对焦虑、恐惧和肠道微生物群的影响之间必须存在联系检查了。了解草甘膦如何影响大鼠肠道共生体、其行为和大脑病理生理学将有助于阐明这种化学物质对焦虑和恐惧的可能作用。我们将给成年大鼠提供受草甘膦污染的饮用水或过滤水，并进行针对焦虑和恐惧相关行为的行为研究。我们假设延长饮用受草甘膦污染的水会导致焦虑和恐惧增加行为。此外，通过评估肠道微生物群组成的变化，我们将识别可能与情绪障碍的发展相关的微生物失衡。