Cryobioprinting for Shelf-Ready Tissue Fabrication and Storage

用于货架组织制造和储存的冷冻生物打印

• 批准号: 10927669
• 负责人: Yu Shrike Zhang
• 金额: $ 49.93万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-19 至 2024-09-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10927669
• 关键词: 3-Dimensional; Address; Adopted; Biological Assay; Cells; Clinical; Collagen; Cryopreservation; Cryopreserved Tissue; Cryoprotective Agents; Engineering; Formulation; Freezing; Future; Gelatin; Goals; Height; Hyaluronic Acid; Hydrogels; Implant; In Situ; In Vitro; Industrialization; Laboratories; Methodology; Methods; Organoids; Outcome; Performance; Printing; Process; Production; Property; Rodent; Sampling; Scheme; Shapes; Site; Structure; Techniques; Technology; Temperature; Testing; Tissue Engineering; Tissue Model; Tissue constructs; Tissues; Trachea; Transplantation Surgery; biofabrication; bioink; bioprinting; cell dimension; chorioallantoic membrane; clinical application; clinical translation; cold temperature; crosslink; cryogenics; design; experimental study; fabrication; in vivo; next generation; preservation; translational potential

Abstract Three-dimensional (3D) bioprinting has paved a new avenue for fabricating intricate cell-laden tissue constructs. So far, bioprinting has been adopted in myriad applications such as tissue engineering, tissue model engineering, and organoid production, among others. There are, however, challenges regarding the fabrication and storage of shelf-ready 3D-bioprinted tissue constructs. Due to the intrinsic complexities involved in most bioprinting processes, including the broadly adopted extrusion bioprinting, using this method as an on- site fabrication technique can be cumbersome, or sometimes impractical under a number of scenarios. Furthermore, the lack of a functional approach for long-term storage of cell-laden tissue constructs precludes the shelf-availability of pre-made bioprinted products. Here, we propose to develop a unique cryobioprinting strategy for simultaneously fabricating and storing cell-laden volumetric tissue constructs through seamlessly combining cell-laden extrusion bioprinting and cryopreservation. The cryobioprinting performances will be investigated by designing, fabricating, and storing cell-laden constructs made of cryoprotective bioinks using a freezing plate with precisely controllable temperature. The in situ freezing process is further anticipated to promote the printability of cell-laden hydrogel bioinks to achieve freeform structures otherwise oftentimes inconvenient with direct extrusion bioprinting. We believe that our cryobioprinting will emerge as a single-step method for concurrent tissue biofabrication and storage.

抽象的 三维（3D）生物打印为制造复杂的充满细胞的组织开辟了一条新途径 构造。迄今为止，生物打印已在组织工程、组织工程、组织工程等众多应用中得到采用。 模型工程和类器官生产等。然而，在以下方面仍存在挑战 制造和储存可上架的 3D 生物打印组织结构。由于所涉及的内在复杂性 在大多数生物打印过程中，包括广泛采用的挤出生物打印，使用这种方法作为 现场制造技术可能很麻烦，或者在许多情况下有时不切实际。 此外，缺乏长期储存充满细胞的组织构建体的功能方法阻碍了 预制生物打印产品的货架可用性。在这里，我们建议开发一种独特的冷冻生物打印 通过无缝同时制造和存储充满细胞的体积组织结构的策略 将充满细胞的挤出生物打印和冷冻保存相结合。冷冻生物打印表演将是 通过使用冷冻保护生物墨水设计、制造和存储充满细胞的结构进行研究 温度精确可控的冷冻板。预计原位冷冻过程将 促进充满细胞的水凝胶生物墨水的可印刷性，以实现自由形式的结构，否则通常会 直接挤出生物打印不方便。我们相信，我们的冷冻生物打印将一步步出现 并发组织生物制造和储存的方法。