Development of stimulated Brillouin microscopy for high-resolution stiffness measurement

开发用于高分辨率刚度测量的受激布里渊显微镜

基本信息

批准号：
10592235
负责人：
Seok-Hyun Andy Yun
金额：
$ 45.8万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10592235
关键词：
3-Dimensional Adoption Affect Area Atomic Force Microscopy Behavior Biocompatible Materials Biological Biological Process Biomechanics Cells Cellular Morphology Cytoskeleton Detection Development Devices Diagnosis Disease Elements Environment Extracellular Matrix Extracellular Space Fostering Frequencies Goals Hardness Health Hydrogels Image In Situ Interferometry Label Lasers Maps Measurement Measures Mechanics Medical Microscope Microscopic Microscopy Mission Modality Modeling Modulus Movement Nature Neoplasm Metastasis Ophthalmologic Surgical Procedures Optics Organism Organoids Phenotype Physiologic pulse Play Process Proliferating Property Public Health Research Resolution Role Sampling Scheme Science Slice Source Spectrum Analysis Speed Stress Surface System Techniques Technology Time Tissues United States National Institutes of Health Work biomechanical test bioscaffold data acquisition disease diagnosis elastography experience extracellular haptics improved in vivo innovation light scattering mechanical load mechanical properties mechanical signal millimeter millisecond molecular scale novel physical science stem cell differentiation stem cell therapy tissue regeneration tool ultrasound waveguide wound healing

项目摘要

Abstract Cells inside tissues and biomaterials receive mechanical cues that affect the cells’ morphology and function. These phenotype changes in turn can remodel the extracellular environment. To understand this intricate interaction, it is fundamentally necessary to measure the mechanical properties of both intracellular and extracellular space in situ. However, this measurement is very difficult by the lack of suitable tools. The goal of this project is to develop novel optical tools based on stimulated Brillouin scattering (SBS) for measuring and mapping stiffness with 3D resolution. The first specific aim is to develop a longitudinal-wave SBS microscope system using a pulsed scheme to achieve sub-millisecond data acquisition speed, 1000-fold improved over spontaneous Brillouin microscopy. The second specific aim is to develop a shear-wave SBS microscope system. Using the coherent nature of SBS, this new modality may measure shear modulus in a broad range from 100 Pa to 1 MPa with subcellular resolution. The third specific aim is to combine the two systems with a common sample stage allowing both longitudinal and shear moduli to be measured from each microscopic volume. The combined microscope will provide unprecedented label-free, high-resolution access to the full compliance or stiffness tensors of cells, tissues, and hydrogels. The successful development of SBS microscopy will have a major impact on many areas that require mechanical characterization of biomaterials, cells, organoids, tissue slices, and small living organisms at high spatial resolution.

抽象的组织和生物材料内的细胞接收影响细胞形态和功能的机械信号。这些表型变化反过来可以重塑细胞外环境，以了解这种复杂的情况。相互作用，从根本上有必要测量细胞内和细胞内的机械性能然而，由于缺乏合适的工具，这种测量非常困难。该项目的目的是开发基于受激布里渊散射（SBS）的新型光学工具来测量第一个具体目标是开发纵波 SBS。使用脉冲方案实现亚毫秒级数据采集速度1000倍的显微镜系统改进自发布里渊显微镜的第二个具体目标是开发剪切波 SBS。利用 SBS 的相干性质，这种新模式可以测量剪切模量。第三个具体目标是将两者结合起来，范围从 100 Pa 到 1 MPa。具有公共样品台的系统允许测量每个样品台的纵向模量和剪切模量组合显微镜将提供前所未有的无标记、高分辨率的访问。细胞、组织和水凝胶的完全顺应性或刚度张量的成功开发。 SBS 显微镜将对许多需要机械表征的领域产生重大影响高空间分辨率的生物材料、细胞、类器官、组织切片和小型活生物体。