Gene Analysis Core

基因分析核心

• 批准号: 10620711
• 负责人: Bruce J Aronow
• 金额: $ 29.74万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10620711
• 关键词: ATAC-seq; Acceleration; Algorithm Design; Benchmarking; Bioinformatics; Biological Assay; Body Fluids; Cell surface; Cells; ChIP-seq; Childhood; Clinical Data; Computational Technique; Computers and Advanced Instrumentation; Consultations; Data; Data Set; Deposition; Digestive System Disorders; Disease; E protein; Educational workshop; Ensure; Environment; Evolution; Experimental Designs; Flow Cytometry; Gene Expression; Generations; Genes; Genetic; Genomics; Goals; Health; Hour; Industry; Infrastructure; Journals; Knowledge; Laboratories; Leadership; Link; Medical center; Medicine; Metadata; Molecular; Monitor; Output; Pediatric Hospitals; Peer Review; Performance; Positioning Attribute; Process; Productivity; Proteins; Protocols documentation; Publications; Publishing; RNA Splicing; Research; Research Personnel; Sampling; Secure; Sequence Analysis; Series; Services; Site; Structure; System; Technology; Tissues; Universities; Validation; base; bench to bedside; cluster computing; college; computational platform; data access; data exploration; data harmonization; data integration; data mining; data quality; data sharing; deep sequencing; epigenomics; experience; experimental study; firewall; genetic analysis; genomic data; human disease; improved; large datasets; member; metadata standards; multiple omics; programs; protein expression; repository; research study; sequencing platform; single cell sequencing; transcriptome sequencing; whole genome

PROJECT SUMMARY The overall goal of the Gene Analysis Core of the Digestive Health Center (DHC) is to provide DHC investigators with fully integrated services to catalyze research on genetics and genomics using advanced instrumentation, reliable protocols, and knowledge for experimental planning and execution. The Core pursues this goal with four complementary aims. In the First Aim “to develop an integrated service structure for studies on gene sequence and function,” the Core integrates a comprehensive portfolio of services into one operational unit with a shared leadership of laboratories that provide gene-based assays and expert bioinformaticians and protein quantification technologies. This structure facilitates consultation and service requests by DHC investigators, with well-defined processes for service request, monitoring of progress, and data sharing. In the Second Aim “To generate genomics data using cutting edge assays to assess gene sequence and function,” the Core makes available to DHC investigators streamlined. In the Third Aim “to provide bioinformatics consultation and analysis for genetics and genomics studies,” the Core gives members opportunities for the optimization of experimental design, technology selection, bioinformatics, and functional annotation of the large datasets produced by the Gene Sequence and Expression Laboratories. Bioinformaticians use refined workflows and computational approaches to fully integrate data from orthogonal technologies, develop improved metadata standards for application in digestive disease datasets at multiple levels of granularity, and provide new solutions for data access and exploration. Bioinformaticians personalize analytical pipelines to meet the investigators’ needs using powerful computing cluster environment and in a secured fashion by industry-quality firewall systems. And in the Fourth Aim to “enable the validation of gene expression at the protein level,” the Core offers investigators the opportunity to validate at the protein level the expression of gene and gene groups using complementary assays in body fluids, cell surface, and intracellular environment. The service portfolio managed by the Gene Analysis Core has been highly subscribed by DHC investigators and has been linked to a high scientific output in peer-reviewed original publications that are relevant to digestive disease.

项目概要 消化健康中心（DHC）基因分析核心的总体目标是提供DHC 研究人员提供完全集成的服务，利用先进的技术来促进遗传学和基因组学研究 The Core 追求的是仪器、可靠的协议以及实验规划和执行的知识。 这个目标有四个互补的目标，即“开发一个综合的研究服务结构”。 “关于基因序列和功能”，核心将全面的服务组合整合为一个 运营单位，由提供基因检测和专家的实验室共同领导 这种结构有利于咨询和服务。 DHC 调查人员提出的请求，具有明确的服务请求流程、进度监控以及 数据共享的第二个目标是“使用尖端分析方法生成基因组学数据来评估基因”。 序列和功能”，该核心在第三个目标中向 DHC 调查人员提供了简化的信息。 为遗传学和基因组学研究提供生物信息学咨询和分析，”核心为成员提供 优化实验设计、技术选择、生物信息学和功能的机会 基因序列和表达实验室产生的大型数据集的注释。 生物信息学家使用精细的工作流程和计算方法来充分整合正交数据 技术，制定改进的元数据标准，用于多个消化系统疾病数据集的应用 粒度级别，并为生物信息学家个性化数据访问和探索提供新的解决方案。 使用强大的计算集群环境和在一个分析管道中满足研究人员的需求 通过行业质量的防火墙系统来保护时尚，第四个目标是“实现基因验证”。 蛋白质水平的表达”，核心为研究人员提供了在蛋白质水平验证 使用体液、细胞表面和细胞内的互补测定法表达基因和基因组 基因分析核心管理的服务组合得到了DHC的高度认可。 研究人员并与同行评审的原始出版物中的高科学产出联系在一起 与消化系统疾病有关。