Indiana Center for Advanced Renal Microscopy and Molecular Imaging

印第安纳高级肾脏显微镜和分子成像中心

• 批准号: 10747616
• 负责人: Pierre C Dagher
• 金额: $ 96.08万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747616
• 关键词: 3-Dimensional; Applications Grants; Biological; Collaborations; Communities; Cytometry; Data; Development; Diabetes Mellitus; Educational workshop; Environment; Equipment; Functional disorder; Funding; Generations; Goals; Hematology; Human BioMolecular Atlas Program; Image; Image Analysis; Imaging technology; Indiana; Individual; Infrastructure; Institution; Kidney; Left; Light; Methods; Microscopy; Mission; Molecular; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Nebraska; Nephrology; Physiology; Publications; Quantitative Microscopy; Rattus; Renal Tissue; Reporter; Research; Research Personnel; Resolution; Resource Development; Resources; Services; Techniques; Technology; Tissues; Training; United States National Institutes of Health; Universities; Washington; Work; biomedical resource; career; design; digital imaging; experience; innovative technologies; intravital microscopy; microscopic imaging; molecular imaging; multi-photon; new technology; novel; outreach; precision medicine; programs; synergism; tool; transcriptomics

Project Summary/Abstract (Center Overview) From its inception as an NIDDK-funded P50 Center in 2002, the mission of the Indiana O’Brien Center for Advanced Renal Microscopy has been to provide renal researchers with effective access to advanced forms of microscopy. Over the past 20 years of continuous funding, the IU O’Brien Center has pioneered the development and dissemination of novel methods of multiphoton intravital microscopy, quantitative tissue cytometry and digital image analysis that have been applied in collaborative studies of renal physiology and pathophysiology with investigators from around the world. Studies conducted with the P30 and P50 IU O’Brien Center have generated data used in over 190 publications and numerous grant applications that have helped launch the careers of a new generation of investigators. We look forward to continuing our work within the consortium framework of the new U54 mechanism. The goals and projects proposed for the Center are organized into three general aims – (1) Provide quantitative intravital microcopy of mice and rats as a service to renal researchers. Provide hands-on training to encourage dissemination of intravital microscopy as an effective research tool. (2) Provide quantitative, large-scale microscopy of the kidney as a service to renal researchers. Three-dimensional confocal tissue cytometry, CODEX multiplexed cytometry and Spatial Transcriptomics will be offered individually or in combination, supporting uniquely comprehensive molecular characterizations. (3) Develop and implement methods of tissue clearing, light-sheet microscopy and digital image analysis to expand the scale of quantitative microscopy of renal tissues, based upon a unique multi- scale/multi-resolution strategy and state-of-the-art methods of digital image analysis. The Indiana O’Brien Center will include an Administrative Core, and two Biomedical Resource cores - an Intravital Microscopy Core, and a Molecular Imaging Core, both hosted by the Indiana Center for Biological Microscopy. Insofar as these two cores effectively extend services currently offered by the Indiana O’Brien Center, they will benefit from the existing infrastructure, expertise and experience developed over the past twenty years by the Center. The Center will also support a Resource Development Core, in collaboration with investigators at the University of Washington, University of Nebraska and Purdue University that will develop technologies that will start to be implemented at the beginning of the second year of funding.

项目总结/摘要（中心概况） 自 2002 年作为 NIDDK 资助的 P50 中心成立以来，印第安纳奥布莱恩中心的使命 先进的肾脏显微镜技术一直致力于为肾脏研究人员提供先进的方法 在过去 20 年的持续资助中，印第安纳大学奥布莱恩中心开创了显微镜技术的先河。 多光子活体显微镜、定量组织新方法的开发和传播 细胞计数和数字图像分析已应用于肾脏生理学的合作研究 使用 P30 和 P50 IU O’Brien 进行的病理生理学研究。 该中心已生成 190 多种出版物和大量资助申请中使用的数据，这些数据帮助 我们期待着在该领域继续我们的工作。 新U54机制的联盟框架为该中心提出的目标和项目是 分为三个总体目标 - (1) 提供小鼠和大鼠的定量活体显微镜检查服务 向肾脏研究人员提供实践培训，以鼓励传播活体显微镜检查作为一种方法。 (2) 提供大规模肾脏显微镜检查作为肾脏服务。 研究人员使用三维共聚焦组织细胞术、CODEX 多重细胞术和空间细胞术。 转录组学将单独或组合提供，支持独特的综合分子 (3) 开发并实施组织透明化、光片显微镜和数字化方法 图像分析扩大了肾组织定量显微镜的规模，基于独特的多 尺度/多分辨率策略和最先进的数字图像分析方法。 印第安纳奥布莱恩中心将包括一个行政核心和两个生物医学资源核心——一个 活体显微镜核心和分子成像核心，均由印第安纳生物中心主办 就这两个核心有效扩展了印第安纳奥布莱恩目前提供的服务而言。 中心，他们将受益于现有的基础设施、过去发展的专业知识和经验 该中心还将与以下机构合作支持资源开发核心。 华盛顿大学、内布拉斯加大学和普渡大学的研究人员将开发 将在资助的第二年年初开始实施的技术。