ShEEP Request for A Namocell PALA Single CellDispenser

ShEEP 请求 Namocell PALA 单细胞分配器

• 批准号: 10739131
• 负责人: Joseph Anthony Garcia
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10739131
• 关键词: Acceleration; Address; Aging; Alzheimer' s Disease; Anemia due to Chronic Disorder; Animal Disease Models; Animal Experiments; Animal Model; Antibodies; Arkansas; Biochemical; Biomedical Research; Blood; CRISPR/Cas technology; Cell Culture Techniques; Cell Line; Cell Separation; Cell Survival; Cell model; Cells; Clone Cells; Cloning; Clustered Regularly Interspaced Short Palindromic Repeats; Contract Services; DNA; Data; Diabetes Mellitus; Disease; Engineering; Equipment; Evaluation; Flow Cytometry; Funding Opportunities; Generations; Genetic; Genetic Engineering; Genetic study; Genomic Library; Glean; Goals; Health; Human; Human Genetics; Industry; Infectious Agent; Kidney; Kidney Diseases; Lasers; Liquid substance; Malignant Neoplasms; Medical; Metabolic; Metabolic Diseases; Microfluidics; Mind; Modeling; Modernization; Modification; Molecular; Mus; N phosphonoacetyl L aspartate; National Institute of Drug Abuse; Nature; Neurodegenerative Disorders; Neurologic; Noise; Organ; Patients; Pharmaceutical Preparations; Population; Proteins; RNA; Recovery; Reproducibility; Research; Research Personnel; Rodent; Rodent Model; Sheep; Shipping; Signal Transduction; Sorting; Spinal cord injury; Structure; Study models; System; Techniques; Therapeutic; Tissues; Training; Transfection; Transformed Cell Line; Traumatic Brain Injury; United States National Institutes of Health; Update; Variant; Veterans; Virus; applied biomedical research; cell transformation; cell type; diet-induced obesity; human disease; human model; improved; induced pluripotent stem cell; innovation; insight; instrument; interest; microfluidic technology; miniaturize; multidisciplinary; multiple omics; novel; operation; precision medicine; preservation; pressure; programs; rapid test; single cell analysis; spinal cord and brain injury; synthetic biology

We are requesting a Namocell PALA Single Cell Dispenser, a benchtop instrument that combines innovative microfluidics technology with flow cytometry and liquid dispensing. It can sort and dispense single cells into 96 or 384-well plates in minutes. Its low-pressure operation (<2 psi) preserves cell viability as well as integrity. The ability to isolate specific cell populations is invaluable for modern basic and applied biomedical research. Microfluidic droplet dispensers provide for miniaturized, high-throughput isolation of single cells in a microenvironment, which can be leveraged for ultra-sensitive and rapid assays. These units are ideal for a wide range of single cell applications including cell cloning with improved outgrowth, generation of high-yield single cell genomics libraries, rapid bulk sorting of specific target cells or rare cell populations, and recovery of single cells from as few as 100 cells. Single cell dispensers are currently in use in several academic and industry settings. Recent acquisition of Namocell Single Cell Dispensers by federally supported facilities include NIH/NIDA and Arkansas Little Rock VA. Data obtained with the PALA Single Cell Dispenser will have applications for both cell culture as well as rodent model studies of human disease. As such, we expect it will aid us in deciphering how these diseases develop or progress as well as help in identifying new treatment opportunities for Veterans with these diseases. The expected research applications for the Namocell PALA Single Cell Dispenser at JJP VAMC will range from bulk sorting of homogenous cell populations to single cell isolation. Bulk sorting is useful for initial interrogations of highly enriched, specific cell types. We expect JJP VAMC investigators who use rodent models of human diseases including those with neurological, renal, metabolic, or cancer relevance will use the PALA for bulk sorting of specific or rare cell populations, particularly those residing in organs with heterogenous cell types [Major Users: JG, CC, GP, RR]. Bulk sorting is also useful for identifying cells manipulated in culture that are marked by fluorescent activity, such as transfected cells expressing specific fluorescent proteins (FP). The dual laser system in the PALA will allow for selection of cells expressing dual exogenous proteins with distinct FP surrogates without the need for prolonged drug selection, which otherwise can introduce confounding factors [Major Users: JG, CC, RR]. The Namocell PALA Single Cell Dispenser will also be used for purification of single cells. Single-cell analysis can discriminate signal from noise in a heterogeneous cell population and thereby facilitate multiomic studies of rare cell populations obtained from tissues or blood of healthy or disease animal models [Major Users: JG, CC, GP, RR]. The PALA will also facilitate high-throughput isolation of single cells in a defined microenvironment, which can be leveraged for ultra-sensitive and rapid assays [Major Users: JG, GP, RR]. Finally, we anticipate great interest in the use of the PALA for cloning of precision genetically engineered transformed cells or induced pluripotent stem cells (iPSC), which may offer insights into structure-function studies as well as human disease contributions from nonsynonymous variants identified in human genetic studies, respectively [Major Users: JG, CC, GP, RR].

我们需要一台 Namocell PALA 单细胞分配器，这是一种台式仪器， 将创新的微流体技术与流式细胞术和液体分配相结合。它可以 在几分钟内将单细胞分选并分配到 96 或 384 孔板中。其低压运行（<2 psi) 保持细胞活力和完整性。分离特定细胞群的能力是 对于现代基础和应用生物医学研究具有不可估量的价值。微流控液滴分配器 提供微环境中单细胞的小型化、高通量分离， 可用于超灵敏和快速的检测。这些装置非常适合各种 单细胞应用，包括改善细胞生长、产生高产量的细胞克隆 单细胞基因组库，快速批量分选特定靶细胞或稀有细胞群， 以及从少至 100 个细胞中回收单个细胞。 单细胞分配器目前在多种学术和工业环境中使用。最近的 联邦政府支持的设施收购 Namocell 单细胞分配器包括 NIH/NIDA 和阿肯色州小石城弗吉尼亚州。使用 PALA 单细胞分配器获得的数据将 可应用于细胞培养以及人类疾病的啮齿动物模型研究。像这样， 我们希望它将帮助我们破译这些疾病是如何发展或进展的，并有助于 为患有这些疾病的退伍军人寻找新的治疗机会。 Namocell PALA 单细胞分配器在 JJP VAMC 的预期研究应用 范围从同质细胞群的批量分选到单细胞分离。批量排序是 对于高度富集的特定细胞类型的初步询问非常有用。我们期待 JJP VAMC 研究人员使用人类疾病的啮齿动物模型，包括神经、肾脏、 代谢或癌症相关性将使用 PALA 对特定或稀有细胞进行批量分选 人群，特别是那些居住在具有异质细胞类型的器官中的人群[主要用户：JG， CC、GP、RR]。批量分选还可用于识别在培养物中进行操作的标记细胞 通过荧光活性，例如表达特定荧光蛋白（FP）的转染细胞。 PALA 中的双激光系统将允许选择表达双外源性的细胞 具有不同 FP 替代物的蛋白质，无需长时间的药物选择，这 否则会引入混杂因素[主要用户：JG、CC、RR]。 Namocell PALA 单细胞分配器也将用于单细胞的纯化。 单细胞分析可以区分异质细胞群中的信号和噪声 从而促进从组织或血液中获得的稀有细胞群的多组学研究 健康或疾病动物模型[主要用户：JG、CC、GP、RR]。 PALA 还将促进 在定义的微环境中对单细胞进行高通量分离，可以利用 用于超灵敏和快速测定[主要用户：JG、GP、RR]。最后，我们期待伟大的 对使用 PALA 克隆精密基因工程转化细胞感兴趣 或诱导多能干细胞（iPSC），这可能为结构功能研究提供见解 以及人类遗传中发现的非同义变异对人类疾病的贡献 分别进行研究[主要用户：JG、CC、GP、RR]。