Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement

蚊子-内共生体-病毒相互作用的代谢基础 RBL 管理补充品

• 批准号: 10631510
• 负责人: Rushika Perera
• 金额: $ 37.01万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631510
• 关键词: Acute; Administrative Supplement; Aedes; Arboviruses; Award; Bacteria; Biocontrols; Biophysics; Blood Vessels; Cell Culture Techniques; Cells; Chikungunya virus; Choking; Country; Culicidae; Data; Dengue Fever; Dengue Hemorrhagic Fever; Dengue Shock Syndrome; Dengue Virus; Diagnostic; Energy Metabolism; Extravasation; Family; Fever; Flaviviridae; Funding; Genome; Goals; Grant; Heritability; Human; In Vitro; Infection; Insecta; Investigation; Laboratories; Life; Life Cycle Stages; Lipids; Lipolysis; Maintenance; Mediating; Membrane; Metabolic; Metabolic Pathway; Methods; Modeling; Modification; National Institute of Allergy and Infectious Disease; Outcome; Parents; Pathway interactions; Persons; Phenotype; RNA Viruses; Research; Resources; Risk; Serotyping; Site; Sphingolipids; Structure; Syndrome; Therapeutic; Tissues; Translations; United States National Institutes of Health; Vaccinee; Vaccines; Viral; Viral Physiology; Viral Proteins; Virion; Virus; Virus Replication; Wolbachia; World Health Organization; Zika Virus; antagonist; biophysical analysis; chikungunya infection; density; endosymbiont; fitness; insight; interest; lipid biosynthesis; metabolic phenotype; metabolome; mitochondrial metabolism; particle; pathogen; success; symbiont; transmission process; vector; vector mosquito; viral transmission

PROJECT SUMMARY / ABSTRACT This supplement is responsive to the Notice of Special Interest, NOT-AI-22-049, “Administrative Supplements for NIAID Regional Biocontainment Laboratories (RBL) and other NIH Grantees Conducting Research at RBL- affiliated Sites” and the scope of our NIAID grant, (fund R01AI151166), entitled, “Metabolic basis of mosquito- endosymbiont-virus interactions.” Here, we will accelerate and advance Specific Aims 1-3 of the parent award through two primary supplementary aims; SA 1-3.supp1: Assess how modification of cellular lipids alters the composition of the viral envelope and required structural transitions for maturation and infectivity in Ae. Aegypti. The data generated from SA 1-3.supp1 will significantly enhance the parent award data outcomes as it provides biophysical insight into how lipid modulation might alter virus structure and function and thus impact the viral life cycle in Ae. aegypti. SA 2.supp2: Assess how Wolbachia-mediated lipid changes alter ZIKV and CHIKV infection and virus-associated lipid modulations. The data generated from SA 2.supp2 will significantly enhance the parent award data outcomes by providing additional insight into the metabolic state of the vector following infection with all three vectored viruses. The studies will enhance our understanding of metabolic choke points that may be applicable to all three viruses, and/or significant differences that might hinder biocontrol efforts. Both efforts synergize with the goals of the RBL in (diagnostics, therapeutics, vaccines, and transmission control methods for Arboviruses vectored by Ae. aegypti); and has the full support of the Scientific Director of the RMRBL.

项目概要/摘要 本补充是对特别利益通知的回应，NOT-AI-22-049，“行政补充 NIAID 区域生物防护实验室 (RBL) 和其他 NIH 受资助者在 RBL 进行研究 附属网站”以及我们 NIAID 赠款的范围（基金 R01AI151166），题为“蚊子的代谢基础- 内共生体-病毒相互作用。”在这里，我们将加速并推进家长奖的具体目标 1-3 通过两个主要补充目标；SA 1-3.supp1：评估细胞脂质的修饰如何改变 埃及伊蚊病毒包膜的组成以及成熟和感染性所需的结构转变。 SA 1-3.supp1 生成的数据将显着增强家长奖励数据结果，因为它提供了 关于脂质调节如何改变病毒结构和功能从而影响病毒生命的生物物理学见解 埃及伊蚊的循环。 SA 2.supp2：评估沃尔巴克氏菌介导的脂质变化如何改变 ZIKV 和 CHIKV 感染 SA 2.supp2 生成的数据将显着增强亲本。 通过提供对感染后载体代谢状态的额外了解来奖励数据结果 所有这三种载体病毒的研究将增强我们对可能的代谢瓶颈的理解。 适用于所有三种病毒，和/或可能阻碍生物防治工作的重大差异。 与 RBL 在（诊断、治疗、疫苗和传播控制方法）方面的目标协同作用 对于以埃及伊蚊为载体的虫媒病毒）；并得到了 RMRBL 科学主任的全力支持。