Administrative supplement to Duke G20 award

杜克大学G20奖的行政补充

• 批准号: 10626469
• 负责人: Colin S. Duckett
• 金额: $ 326.48万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-23 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626469
• 关键词: Address; Administrative Supplement; Air; Animal Experimentation; Animal Model; Automation; Award; Basic Science; Biological Assay; Cells; Computer Security; Containment; Contractor; Contracts; Diagnostic; Equipment; Facility Design and Construction; Facility Designs; Fluorescence Microscopy; Freezing; Funding; Goals; Grant; Incubators; Infection; Infrastructure; Laboratories; Laboratory Research; Liquid substance; Modernization; Monitor; Mus; Parents; Pharmaceutical Preparations; Reader; Recovery; Research; Research Personnel; Resources; Security; Service provision; Services; Solid; System; Time; Trust; United States National Institutes of Health; Universities; Vaccines; Vendor; aged; animal imaging; biocontainment facility; biodefense; cost; innovation; laboratory equipment; laboratory facility; medical schools; microCT; next generation; operation; repaired

Project Summary/Abstract: The Duke Regional Biocontainment Laboratory (RBL) was constructed with funding from the National Institutes of Health (NIH) to support basic research necessary to develop drugs, diagnostics, and vaccines for emerging/reemerging infections and biodefense. The state-of-the-art biocontainment facility, designed to support lab and small animal research, was fully commissioned and opened for operation in 2007 on the Duke University Medical School Campus in Durham, NC. The Duke RBL facility supports grant/contract-funded investigators and also provides biocontainment labs, innovative host monitoring assays and small animal models through Core Service Center Units on a cost recovery basis. Fourteen years of use and time have rendered some of the Duke RBL facilities, systems and critical core research equipment obsolete, or in need of repair, replacement or modernization. Since award of the parent G20 we have identified additional critical deficiencies of the facility’s existing physical infrastructure that limit biosafety, security or threaten the provision of services it offers and research-related activities it supports. We will address these additional deficiencies through this supplement funding in our three original Specific Aims: 1) Modernize Building Control Systems – Replace and upgrade obsolete air compressor and the BACNet (Building Automation Control Network); 2) Modernize Biosafety and Security Systems - Replace aged/damaged PAPR units; and 3) Modernize Research Resources – Replace or add additional major equipment to the Duke RBL Research Service Cores - liquid handling automation equipment, ELISpot reader, next-generation Luminex reader, upgraded cell counter, replacement incubators, freezers and centrifuges, advanced fluorescence microscopy capability in BSL3, additional Allentown Biocontainment mouse caging, and live animal imaging with micro CT capability. These Aims/Goals will be accomplished by the highly integrated project management team assembled in the parent award with solid institutional support and trusted contractors/vendors that were part of the original design and construction of the facility.

项目摘要/摘要： 杜克大学地区生物防护实验室 (RBL) 是在美国国立研究院的资助下建造的 卫生署 (NIH) 支持开发药物、诊断方法和疫苗所需的基础研究 新出现/重新出现的感染和生物防御。最先进的生物防护设施，旨在 支持实验室和小动物研究，于 2007 年在杜克大学全面投入运行并开放运营 北卡罗来纳州达勒姆大学医学院校园杜克大学 RBL 设施支持赠款/合同资助。 研究人员还提供生物防护实验室、创新的宿主监测分析和小动物模型 通过核心服务中心单位在成本回收的基础上已经使用了十四年。 杜克大学 RBL 的一些设施、系统和关键核心研究设备已经过时或需要维修， 自授予母公司 G20 以来，我们发现了其他关键缺陷。 设施现有的有形基础设施限制了生物安全、安保或威胁其提供的服务 我们将通过此解决这些额外的缺陷。 补充资金以实现我们最初的三个具体目标：1) 现代化楼宇控制系统 – 更换和 升级陈旧的空气压缩机和 BACNet（楼宇自动化控制网络）；2) 现代化； 生物安全和安保系统 - 更换老化/损坏的 PAPR 装置，以及 3) 实现研究资源现代化； – 为杜克大学 RBL 研究服务核心更换或添加额外的主要设备 - 液体处理 自动化设备、ELISpot 读数器、下一代 Luminex 读数器、升级的细胞计数器、更换 培养箱、冷冻机和离心机、BSL3 中的先进荧光显微镜功能、额外的阿伦敦 这些目的/目标将是生物防护小鼠笼和具有微型 CT 功能的活体动物成像。 由母公司组建的高度集成的项目管理团队完成，具有扎实的 机构支持和值得信赖的承包商/供应商是最初设计和建造的一部分 设施。

项目成果

A Virion-Based Combination Vaccine Protects against Influenza and SARS-CoV-2 Disease in Mice.

基于病毒粒子的联合疫苗可预防小鼠流感和 SARS-CoV-2 疾病。

• 发表时间: 2022-08-10
• 作者: Chaparian, Ryan R;Harding, Alfred T;Hamele, Cait E;Riebe, Kristina;Karlsson, Amelia;Sempowski, Gregory D;Heaton, Nicholas S;Heaton, Brook E
• 通讯作者: Heaton, Brook E

Transient inhibition of lysosomal functions potentiates nucleic acid vaccines.

溶酶体功能的瞬时抑制可增强核酸疫苗的作用。

• 发表时间: 2023-10-31
• 影响因子: 11.1
• 作者: Wang, Chunxi;Karlsson, Amelia;Oguin 3rd, Thomas H;Macintyre, Andrew N;Sempowski, Gregory D;McCarthy, Kevin R;Wang, Yifei;Moody, M Anthony;Yuan, Fan
• 通讯作者: Yuan, Fan

H3N2 influenza hemagglutination inhibition method qualification with data driven statistical methods for human clinical trials.

H3N2 流感血凝抑制方法的鉴定，采用数据驱动的统计方法进行人体临床试验。

• 发表时间: 2023
• 作者: Sawant, Sheetal;Gurley, Sarah Anne;Overman, R Glenn;Sharak, Angelina;Mudrak, Sarah V;Oguin 3rd, Thomas;Sempowski, Gregory D;Sarzotti;Walter, Emmanuel B;Xie, Hang;Pasetti, Marcela F;Moody, M Anthony;Tomaras, Georgia D
• 通讯作者: Tomaras, Georgia D

Targeted dose delivery of Mycobacterium tuberculosis in mice using silicon antifoaming agent via aerosol exposure system.

使用硅消泡剂通过气溶胶暴露系统对小鼠结核分枝杆菌进行靶向剂量递送。

• 发表时间: 2022
• 影响因子: 3.7
• 作者: Gautam, Uma Shankar;Asrican, Rosemarie;Sempowski, Gregory D
• 通讯作者: Sempowski, Gregory D