Mechanism of nerve growth factor driven axon plasticity

神经生长因子驱动轴突可塑性机制

• 批准号: 10626679
• 负责人: GIANLUCA GALLO
• 金额: $ 39.63万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10626679
• 关键词: Ablation; Actins; Active Biological Transport; Address; Afferent Neurons; Axon; Bioenergetics; Biology; Complex; Cytoskeleton; Data; Development; Distal; Embryo; Energy-Generating Resources; Enzymes; Fibrinogen; Filopodia; Generations; Glycolysis; Growth Cones; Image; Impairment; In Vitro; Link; Maintenance; Microfilaments; Microtubules; Mitochondria; Molecular; Morphogenesis; Morphology; Nerve Growth Factors; Neurons; Oxidative Phosphorylation; Pathway interactions; Pharmacology; Phase; Phosphorylation; Physiological; Polymers; Process; Production; Publishing; Regulation; Reporting; Rest; Role; Sensory; Signal Transduction; Spinal Cord; Techniques; Testing; Work; base; enzyme pathway; experimental study; fast axonal transport; imaging approach; mutant; neuron development; neuronal circuitry; neurotrophic factor; optogenetics; presynaptic; repaired; response; spatiotemporal

PROJECT SUMMARY/ABSTRACT Axon plasticity, in the form of the generation of axon branches, is fundamental to the development and repair of neuronal circuitry. In this project we will address the main hypothesis that the intra-axonal glycolytic pathway is required for the formation and maintenance of axon branches in vitro and in vitro. Live imaging approaches, molecular manipulations, pharmacology and optogenetic ablation of glycolytic enzymes will be used to determine the role of the glycolytic pathway. The project will also address the link between the glycolytic pathway and the cytoskeletal remodeling underlying axon branching.

项目概要/摘要 轴突可塑性，以轴突分支生成的形式，对于轴突分支的形成至关重要。 神经回路的发育和修复。在这个项目中，我们将解决以下主要假设： 轴突内糖酵解途径是轴突分支的形成和维持所必需的 体外和体外。实时成像方法、分子操作、药理学和光遗传学 糖酵解酶的消融将用于确定糖酵解途径的作用。项目 还将解决糖酵解途径和潜在的细胞骨架重塑之间的联系 轴突分支。