Core B - The Clinical and Biomarkers Core

核心 B - 临床和生物标志物核心

• 批准号: 9149446
• 负责人: DOROTHY K HATSUKAMI
• 金额: $ 48.12万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2021-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9149446
• 关键词: 1-Butanol; 2-butenal; Abbreviations; Acetylcysteine; Acids; Acrolein; Aromatic Polycyclic Hydrocarbons; Biological; Biological Assay; Biological Availability; Biological Markers; Butanones; Cadmium; Cancer Etiology; Carcinogens; Cessation of life; Cigarette; Clinical; Clinical Trials; Collection; Communities; Cotinine; DNA Adducts; Data; Deoxyguanosine; Deuterium; Development; Diagnostic; Ethnic group; Exposure to; Future; Glucuronides; Goals; Hawaiian population; Human; Human Resources; Individual; Inflammation; Intake; Japanese American; Label; Lead; Lung; Malignant neoplasm of lung; Mass Spectrum Analysis; Measurement; Measures; Metabolism; Methodology; Methods; Nicotine; Oxidative Stress; Play; Preventive measure; Procedures; Program Research Project Grants; Protocols documentation; Recruitment Activity; Research; Research Personnel; Role; Sampling; Services; Singapore; Smoker; Smoking; Source; Tobacco; United States; base; biobank; cancer epidemiology; cancer risk; case control; cigarette smoking; clinical biomarkers; cohort; ethnic difference; experience; insight; metabolic profile; phenanthrene; programs; propylene oxide; racial and ethnic; racial difference; sample collection; stable isotope; success; tobacco exposure; toxicant; urinary

ABSTRACT The Clinical and Biomarkers Core is critical for the studies in this program project renewal proposal. The Core will recruit smokers to collect biological samples and will carry out analyses of tobacco constituent biomarkers. The biological samples collected by the Core will be used to evaluate and compare biomarkers of tobacco exposure across Japanese Americans, Whites, and Native Hawaiians in Projects 2, 3, and 4. Biomarkers to be analyzed by the Core include total nicotine equivalents (nicotine intake), 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanol (NNAL) and its N- and O-glucuronides (exposure to tobacco-specific lung carcinogen NNK), phenanthrene tetraol and 3-hydroxy phenanthrene (exposure to and metabolism of the representative polycyclic aromatic hydrocarbon phenanthrene), mercapturic acids (exposure to acrolein, crotonaldehyde, and propylene oxide), urinary cadmium, as well as urinary biomarkers and DNA adducts derived from oxidative stress and inflammation. The measurements will be carried out on samples from a total of 6,084 subjects, with some of these samples being analyzed for only a subset of biomarkers. The samples from these subjects will either be procured from the existing bioresposity of the Multiethnic Cohort or obtained from smokers who are recruited by this Core. All assays, except for urinary cadmium, are based on stable-isotope dilution mass spectrometry and all have been analytically validated with respect to accuracy and precision. Personnel in this Core have extensive experience in clinical trials and quantitation of urinary tobacco biomarkers and DNA adducts, and were the first to develop many of the methods that will be used in this program project grant. The biological samples and biomarker results generated by this Core will be used by all projects.

抽象的 临床和生物标志物核心对于该计划项目更新提案中的研究至关重要。核心 将招募吸烟者收集生物样本并对烟草成分生物标志物进行分析。 核心收集的生物样本将用于评估和比较烟草的生物标志物 项目 2、3 和 4 中日裔美国人、白人和夏威夷原住民的暴露情况。生物标志物 核心分析包括总尼古丁当量（尼古丁摄入量）、4-(甲基亚硝基氨基)-1-(3-吡啶基)-1- 丁醇 (NNAL) 及其 N- 和 O-葡萄糖醛酸苷（接触烟草特有的肺癌致癌物 NNK）， 菲四醇和 3-羟基菲（代表性物质的接触和代谢 多环芳烃菲）、硫醇酸（接触丙烯醛、巴豆醛和 环氧丙烷）、尿镉以及尿生物标志物和源自氧化的 DNA 加合物 压力和炎症。测量将对总共 6,084 名受试者的样本进行，其中 其中一些样本仅分析一部分生物标志物。来自这些受试者的样本将 要么从多种族队列的现有生物反应性中获得，要么从吸烟者那里获得 由该核心招募。除尿镉外，所有测定均基于稳定同位素稀释质量 光谱测定法以及所有这些都已在准确性和精密度方面进行了分析验证。这方面的人员 核心在尿烟草生物标志物和 DNA 的临床试验和定量方面拥有丰富的经验 加合物，并且是第一个开发将在该计划项目拨款中使用的许多方法的人。这 该核心生成的生物样本和生物标志物结果将被所有项目使用。