Mass Spectrometry / Proteomics Shared Facility

质谱/蛋白质组学共享设施

• 批准号: 9296325
• 负责人: James A Mobley
• 金额: $ 0.87万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9296325
• 关键词: Amino Acids; Animals; Basic Science; Biological; Cancer Center; Cells; Clinical Research; Collaborations; Complex; Comprehensive Cancer Center; Data; Data Set; Development; Education and Outreach; Experimental Designs; Faculty; Formalin; Freezing; Fresh Tissue; Funding; Global Change; Goals; Grant; Human Development; Individual; Informatics; Liquid substance; Manuscripts; Mass Spectrum Analysis; Molecular Analysis; Mus; Paraffin Embedding; Pathway interactions; Play; Post-Translational Protein Processing; Preparation; Procedures; Process; Proteins; Proteomics; Reagent; Research Support; Resources; Role; Sampling; Services; Stable Isotope Labeling; Systems Biology; Technology; Tissues; Training; Training and Education; Translating; Translations; University of Alabama at Birmingham Cancer Center; Validation; Work; abstracting; anticancer research; base; biomarker discovery; cancer therapy; clinical application; cost effective; disease diagnosis; improved; insight; meetings; member; operation; programs; response; structural biology; treatment response; web site

MOLECULAR ANALYSIS / TRANSLATION GROUP MASS SPECTROMETRY / PROTEOMICS SHARED FACILITY (MSPSF) ABSTRACT The over-arching goal of the Mass Spectrometry/Proteomics Shared Facility (MSPSF) is to provide state-of- the-art capabilities and training in mass spectrometry, proteomics, and bioanalytical technologies to support the research needs of UAB Comprehensive Cancer Center (UAB-CCC) members. The MSPSF is a research resource that provides services to UAB-CCC members that support the identification, characterization and quantification of proteins, as well as post-translational modifications. The proteins may be isolated from cells, biological fluids and tissues (fresh, frozen or formalin-fixed paraffin-embedded [FFPE]). The MSPSF has streamlined and standardized its operations and offers cost-effective, high quality services to UAB-CCC members through utilization of standardized workflows and operating procedures that cover a wide range of applications including the identification of specific post-translational changes, global and targeted quantitative proteomics, and structural biology. The interface between data gathering and analysis has been strengthened and now includes basic and advanced informatics applications. In the current funding cycle, the MSPSF has expanded its services in response to the emerging needs and thematic emphasis of the UAB-CCC through initiatives undertaken in collaboration with other UAB-CCC Shared Facilities. These include the development and validation of robust proteomic analysis carried out using FFPE tissues from mouse and human and development of reagents and animals that permit utilization of approaches based on stable isotope-labeled amino acids in mice (SILAM). The MSPSF supports basic and clinical research within all six programs of the UAB-CCC that is focused on mass spectrometry/proteomics analyses of proteins in either purified or complex biological samples. During the current funding cycle 48 UAB-CCC members utilized the MSPSF to further their cancer research. Information gained provides critical insights that include biomarker discovery for disease diagnosis, progression, and therapeutic response; including changes in cellular pathways associated with a specific process. The services provided play an important role in translating basic science discoveries to clinical applications in the treatment of cancer.

分子分析 /翻译组 质谱 /蛋白质组学共享设施（MSPSF） 抽象的 质谱/蛋白质组学共享设施（MSPSF）的总体目标是提供最新 质谱，蛋白质组学和生物分析技术的ART功能和培训以支持 UAB综合癌症中心（UAB-CCC）成员的研究需求。 MSPSF是一项研究 为支持标识，表征和 蛋白质的定量以及翻译后修饰。蛋白质可以从细胞中分离出来， 生物流体和组织（新鲜，冷冻或福尔马林固定石蜡填充的[FFPE]）。 MSPSF具有 简化和标准化其运营，并为UAB-CCC提供具有成本效益的高质量服务 通过利用标准化工作流程和操作程序的成员 应用程序包括识别特定的翻译后变化，全球和有针对性的定量 蛋白质组学和结构生物学。数据收集和分析之间的接口已得到加强 现在包括基本和高级信息学应用程序。在当前的资金周期中，MSPSF具有 通过响应UAB-CCC的新兴需求和主题强调，扩大其服务 与其他UAB-CCC共享设施合作采取的计划。这些包括发展 以及使用小鼠和人类的FFPE组织进行的鲁棒蛋白质组学分析的验证 开发允许基于稳定同位素标记的方法利用的试剂和动物 小鼠中的氨基酸（Silam）。 MSPSF支持UAB-CCC的所有六个计划中的基础和临床研究，该研究重点是 纯化或复杂的生物样品中蛋白质的质谱/蛋白质组学分析。在 当前的资金周期48 UAB-CCC成员利用MSPSF进一步进一步进行癌症研究。信息 获得的提供了关键见解，包括用于疾病诊断，进展和的生物标志物发现 治疗反应；包括与特定过程相关的细胞途径的变化。服务 提供在将基础科学发现转化为治疗中的临床应用中起着重要作用 癌症。