Spiral tube assembly for countercurrent chromatography: tubing modification by a simple tool to improve the partition efficincy

用于逆流色谱的螺旋管组件：通过简单工具对管道进行改造以提高分配效率

• 批准号: 9353111
• 负责人: Yoichiro Ito
• 金额: $ 113.41万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9353111
• 关键词: Chromatography; Dyes; Goals; Image; Modification; Natural Products; Nucleic Acids; Peptides; Phase; Polymers; Proteins; Resolution; Sampling; Shapes; Solvents; Speed; System; Testing; Tube; Width; aqueous; countercurrent chromatography; design; improved; novel; tool

High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency a simple tool to modify the tubing shapes was fabricated, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flattened, flat-waving, and flat-twisting. Partition efficiencies of the separation column made from these modified tubing were examined in protein separation with an aqueous-aqueous polymer phase system at flow rates of 1-2 ml/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 ml/min, but at a higher flow rate of 2 ml/min the columns made of flattened tubing showed lowered partition efficiency apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twisting improve the partition efficiency in a semi-preparative separation while other modified tubing of flattened and flat-waving configurations may be used for analytical separations with a low flow rate.

带有螺旋管组件的高速逆流色谱法可以保留用于蛋白质分离的聚合物相系统的令人满意的固定相。 为了提高分区效率，制造了一个简单的修改管道形状的工具，并进行了以下四个不同的管道修饰：以10 mm的宽度，扁平，平坦的波和平坦的扭动，间歇性地按下。 通过这些修饰的管道制成的分离柱的分区效率在蛋白质分离中使用水性水性聚合物相位系统以1-2 ml/min的流速在800 rpm下进行。 结果表明，所有修饰管的色谱柱以1 ml/min的流速提高了分区效率，但在较高的流速为2 ml/min的流速下，由扁平管制成的色谱柱显然显示出降低的分配效率，这显然是由于保留平稳相的损失。在所有修改的列中，具有间歇性压管的圆柱给出了最佳的峰值分辨率。 可以得出的结论是，间歇性压力和平坦的扭动提高了半程分离中的分区效率，而其他修饰的扁平和扁平效率的管道则可以用于低流速的分析分离。

项目成果

Enantioseparation of mandelic acid derivatives by high performance liquid chromatography with substituted β-cyclodextrin as chiral mobile phase additive and evaluation of inclusion complex formation.

• DOI: 10.1016/j.jchromb.2014.05.026
• 发表时间: 2014-07-01
• 期刊: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
• 影响因子: 3
• 作者: Tong, Shengqiang;Zhang, Hu;Shen, Mangmang;Ito, Yoichiro;Yan, Jizhong
• 通讯作者: Yan, Jizhong

Development of a method to extract and purify target compounds from medicinal plants in a single step: online hyphenation of expanded bed adsorption chromatography and countercurrent chromatography.

• DOI: 10.1021/ac4035955
• 发表时间: 2014-04-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Li, Yang;Wang, Nan;Zhang, Min;Ito, Yoichiro;Zhang, Hongyang;Wang, Yuerong;Guo, Xin;Hu, Ping
• 通讯作者: Hu, Ping

Rapid preparative separation of six bioactive compounds from Gentiana crassicaulis Duthie ex Burk. using microwave-assisted extraction coupled with high-speed counter-current chromatography.

• DOI: 10.1002/jssc.201300897
• 发表时间: 2013-12
• 期刊: JOURNAL OF SEPARATION SCIENCE
• 影响因子: 3.1
• 作者: Liang, Jinru;Ito, Yoichiro;Zhang, Xinxin;He, Jiao;Sun, Wenji
• 通讯作者: Sun, Wenji

Chiral magnetic microspheres purified by centrifugal field flow fractionation and microspheres magnetic chiral chromatography for benzoin racemate separation.

• DOI: 10.1016/j.chroma.2013.07.044
• 发表时间: 2013-08-30
• 期刊: Journal of chromatography. A
• 作者: Tian A;Qi J;Liu Y;Wang F;Ito Y;Wei Y
• 通讯作者: Wei Y

Enrichment and purification of pedunculoside and syringin from the barks of Ilex rotunda with macroporous resins.

• DOI: 10.1080/10826076.2012.749499
• 发表时间: 2014-02-01
• 期刊: Journal of liquid chromatography & related technologies
• 影响因子: 1.3
• 作者: Wang C;Chao Z;Sun W;Wu X;Ito Y
• 通讯作者: Ito Y