IN VIVO IDENTITY AND NICHE OF PERIODONTAL LIGAMENT STEM CELLS

牙周韧带干细胞的体内特性和生态位

• 批准号: 9033038
• 负责人: Hu Zhao
• 金额: $ 12.72万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-02-05 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9033038
• 关键词: Address; Adult; Affect; Apical; Behavior; Biological; Biology; California; Cell Therapy; Cells; Characteristics; Clinic; Clinical; Data; Dental Cementum; Differentiation Antigens; Differentiation and Growth; Environment; Funding; Future; Goals; Gold; Homeostasis; Image; In Vitro; Incisor; Injury; Label; Lead; Mentored Research Scientist Development Award; Mentors; Mesenchyme; Molecular Biology; Molecular Profiling; Natural regeneration; Operative Surgical Procedures; Orthodontic; Pattern; Periodontal Diseases; Periodontal Ligament; Periodontium; Physiological; Play; Process; Property; Research; Research Personnel; Research Training; Resources; Role; Scientist; Signal Pathway; Signal Transduction; Stem Cell Research; Stem cells; Surgical sutures; Testing; Tissue Engineering; Tissues; Tooth Loss; Tooth Movement; Tooth Tissue; Tooth structure; Training; Training Programs; Transgenic Mice; Translating; Universities; Work; alveolar bone; base; bone; bone turnover; career; conventional therapy; craniofacial; experience; gain of function; improved; in vivo; injury and repair; loss of function; mouse model; neurovascular; novel therapeutics; public health relevance; stem cell biology; tissue regeneration

 DESCRIPTION (provided by applicant): The periodontal ligament (PDL) is the fibrous tissue connecting the teeth and the alveolar bone and is critical for the physiological functions of teeth Periodontal diseases are the most common reasons of tooth loss. Conventional treatments including surgical or non-surgical approaches yield limited effects on regenerating pathological periodontal tissue. Recent progress in stem cell biology has led to a promising novel therapy based on the ability PDL-derived stem cells to regenerate functioning periodontium. However, the further improvement of the stem cells based therapies are greatly impeded by the poor understanding of the PDL stem cells in vivo. Although in vitro studies have shown that multipotential stem cells can be isolated from the PDL and it was speculated that the PDL stem cells are crucial for multiple processes including tissue regeneration after injury or diseases, periodontal tissue turnover and orthodontic tooth movement. However, there was virtually no information about the in vivo localization, identity or regulating niche of the PDL stem cells. In our previous study, we have identified Gli1+ cells surrounding the neurovascular bundle as the MSCs to support the incisor mesenchyme turnover and injury repair [7]. The Wnt signaling pathway plays crucial roles on activating the quiescent Gli1+ MSCs. Furthermore, we also identified Gli1+ cells in the craniofacial bone sutures as the MSCs supporting craniofacial bones turnover and injury repair. In the current research, we propose to test the hypothesis that PDL stem cells in the adult are localized near the apical region of the teeth and can be labeled with Gli1. These Gli1+ stem cells give rise to the PDL, cementum and alveolar bone. They support both natural turnover and injury repair of the PDL tissue and are regulated by Wnt signaling in vivo. This application also lays out a five-year research and training program with the ultimate goal to transition myself to an independent clinical scientist working on stem cell research. This funding will provide me with concepts, data, training and experience for future funded independent research. My mentors are leaders in the fields of craniofacial biology, stem cell biology and biological imaging. I will take advantage of the rich resources of the environment in the Center for Craniofacial Molecular Biology of University of Southern California to prepare myself for an independent research career.

 描述（由申请人证明）：牙周韧带（PDL）是牙槽骨骼，对牙齿牙周疾病的生理功能至关重要。 Yieeld对新型PDL衍生的干细胞的影响有限。损伤或疾病后的组织再生，牙周齿状牙齿运动和调节PDL干细胞的生态[7] 1+ MSC。 ETH的顶端可以用Gli1贝尔（Gli1）贝尔（Gli1）染色。通过为我提供概念，数据，培训和未来的经验，将自己的目的过渡到独立的临床科学家。南加州大学的颅面分子生物学，为自己的独立研究职业做好准备。