Omics and Bioinformatics Facility Core

组学和生物信息学设施核心

• 批准号: 9058300
• 负责人: Maureen Agnes Sartor
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9058300
• 关键词: Address; Alternative Splicing; Bioinformatics; Biological; Biological Assay; Cell Count; ChIP-seq; Chromatin; Communities; Complex; Core Facility; Custom; DNA Methylation; Data; Data Set; Databases; Development; Disease; Educational workshop; Environmental Exposure; Environmental Health; Epigenetic Process; Faculty; Funding; Gene Expression; Gene Targeting; Generations; Genes; Genetic; Genetic Transcription; Genome; Genomic Segment; Genomics; Grant; Individual; Life; Link; Manuscripts; Methodology; Methods; Michigan; MicroRNAs; Mission; Molecular; Molecular Conformation; Paraffin Embedding; Pathway Analysis; Pathway interactions; Phenotype; Policies; Preparation; Process; Proteins; Proteomics; Protocols documentation; Publications; Recommendation; Research; Research Design; Research Personnel; Resources; Risk; Sampling; Scientist; Services; Signal Pathway; Staging; Students; Techniques; Technology; Training; Transcript; Untranslated RNA; base; bead chip; bisulfite sequencing; cost; data management; data sharing; data visualization; design; epigenomics; genetic variant; genome-wide; genome-wide analysis; health science research; high throughput technology; histone modification; innovation; meetings; member; metabolomics; new technology; next generation sequencing; novel; protein metabolite; protocol development; pyrosequencing; ranpirnase; sample collection; tool; toxicant; transcription factor; transcriptome sequencing; transcriptomics

ABSTRACT (Omics and Bioinformatics Core) Applications of next generation sequencing, protocols for targeted omics assays, and the number and breadth of publicly-available omics datasets all continue to expand at a rapid pace. As such, it is difficult for environmental health scientists to stay up-to-date on all of the technologies, analysis methods, and available data relevant to their research. The objective of the Omics & BioInformatics Core (OBIC) is to provide comprehensive and innovative support to investigators in the Michigan Center on Lifestage Environmental Exposures and Disease (M-LEEaD) for the study design, sample preparation, analysis, interpretation, and integration of a broad range of omics-based studies, and to educate them on available opportunities. OBIC streamlines the entire process of an omics study, allowing center investigators to dedicate more of their own resources to answering difficult environmental health science questions. Lifestage environmental exposures may increase the risk of later disease through a variety of molecular mechanisms, including epigenetic, genetic, transcriptomic, and metabolomics mechanisms. Technologies for which we provide expertise and analysis include pyrosequencing and Sequenom, RNA-seq, gene expression and DNA methylation microarrays, microRNA expression, HumanMethylation450 BeadChip, reduced representation bisulfite sequencing (RRBS) and MeDIP-seq, and ChIP-seq. The OBIC objective will be accomplished through four specific aims. First, we will provide M-LEEaD investigators expertise in the design and implementation of a broad range of omics and gene target studies (e.g. genomic, epigenomic, proteomic, metabolomic) and development of protocols, including technology platform choice, and sample collection and preparation. Second, we will provide M-LEEaD investigators with a broad-range of bioinformatics analysis services, including standard or custom analyses, support for data management and visualization, and pathway/network analyses. Third, OBIC supports the community through development of novel bioinformatics methods and tools and protocols for novel technologies staying apace with technological advancements. Finally, OBIC educates center faculty, staff, and students on omics assays and bioinformatics methods most relevant to environmental health sciences research, and connects environmental scientists with potential collaborators in omics and bioinformatics. M-LEEaD investigators benefit from free and/or reduced cost services. Our services include assistance in grants and manuscript preparation, individual training on the use of tools and resources, and Bioinformatics/Omics workshops targeted to M-LEEaD investigators.

摘要（OMICS和生物信息学核心） 下一代测序的应用，针对目标分析的协议以及数量和广度 公共可用的OMIC数据集的所有内容都以快速的速度继续扩展。因此，很难 环境卫生科学家将在所有技术，分析方法和可用的所有技术上保持最新状态 与他们的研究相关的数据。 OMICS与生物信息学核心（OBIC）的目的是提供 密歇根州生命环境环境中心的调查员的全面和创新支持 用于研究设计，样本制备，分析，解释和 整合了广泛的基于OMIC的研究，并教育他们的机会。奥贝克 简化了OMICS研究的整个过程，允许中心调查人员更多地专门 回答困难的环境健康科学问题的资源。 Lifestage环境暴露 可能会通过多种分子机制（包括表观遗传学）增加后来疾病的风险 遗传，转录组和代谢组学机制。我们提供专业知识的技术和 分析包括焦磷酸测序和序列，RNA-seq，基因表达和DNA甲基化 微阵列，microRNA表达，人甲基化450 beadchip，降低表示BiSulfite 测序（RRB）和Medip-Seq和Chip-Seq。 OBIC目标将通过四个实现 具体目标。首先，我们将在设计和实施的设计和实施方面为M-Leead调查人员提供专业知识 大量的OMIC和基因靶研究（例如基因组，表观基因组，蛋白质组学，代谢组）和 制定协议，包括技术平台选择以及样本收集和准备。 其次，我们将为M-Leead调查人员提供广泛的生物信息学分析服务， 包括标准或自定义分析，支持数据管理和可视化以及途径/网络 分析。第三，OBIC通过开发新颖的生物信息学方法和工具来支持社区 和新技术的方案，使技术进步保持迅速发展。最后，奥比奇教育 中心教职员工和学生在OMICS分析和生物信息学方法上与环境最相关的方法 健康科学研究，并将环境科学家与OMIC的潜在合作者联系起来 生物信息学。 M-Leead调查人员受益于免费和/或降低的成本服务。我们的服务包括 协助赠款和手稿准备，有关使用工具和资源的个人培训以及 针对M-Leead研究人员的生物信息学/OMICS研讨会。