Profiling and Mapping Core

分析和映射核心

基本信息

批准号：
10271480
负责人：
DAVID Branch MOODY
金额：
$ 45.07万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2026-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10271480
关键词：
Archives Bacteria Bacteriology Biochemical Bioinformatics Biological Biological Assay CRISPR interference Catalogs Cell model Chemical Structure Chemicals Clinical Communities Data Set Databases Detection Development Diagnostic Diagnostics Research Disease Escherichia coli Eukaryotic Cell Genes Genetic Genomics Goals Hour Housekeeping Human Immune response Infectious Diseases Research Investigation Investigational Drugs Laboratories Left Link Lipids Maps Mass Spectrum Analysis Measurement Measures Membrane Membrane Lipids Metabolic Metabolism Methods Modernization Mycobacterium tuberculosis Names Organism Patients Penetration Permeability Pharmaceutical Preparations Physiology Prevalence Research Research Personnel Sampling Series South Africa South African South America Structure System Systems Biology Time Tuberculosis Variant Virulence Whole Organism aqueous base bioinformatics resource community setting comparative computing resources gene function lipidome lipidomics member metabolome metabolomics mycobacterial mycolate operation pathogen rapid technique tool

项目摘要

Profiling and Mapping Core B Project Leaders: Kyu Rhee and D. Branch Moody Co-investigators: Jacob Mayfield ABSTRACT - Profiling and Mapping Core B Modern infectious disease research relies fundamentally on genomic maps of the major pathogens. With the recent development of whole-organism metabolomics profiling tools, comprehensively mapping the M. tuberculosis (Mtb) metabolome is now feasible. Core B supports mass spectrometry-based profiling of Mtb metabolites to support Projects 1, 2 and 3 that focus on discovery, drugs and diagnostics, respectively. The Core will carry out whole-organism lipidomic and metabolomic profiling, archive datasets and use modern bioinformatic methods to generate metabo-lipidomic maps of Mtb. Maps are comprised of a structured listing of all metabolite subclasses, a list of all named metabolites within each class, and links of all named metabolites to mass values and other biological variables. We previously generated the MycoMap Database, which is the largest existing map of 183 mycobacterial metabolite classes linked to ~195,000 accurate mass values. We will now expand the database by adding newly discovered lipids generated in Projects 1, 2 and 3. Supporting Projects 1 and 2, we will solve the Mycolate Outer Membrane Lipid Map, which describes compounds located at the host-pathogen barrier and involved in drug penetration. Supporting Projects 1 and 3, the core will measure metabolite variation among Mtb strains from South African patients to generate the Human Mtb Strain Lipid Variation Database. This database will describe the prevalence and variation of lipids among strains circulating in human communities to identify lipids under control by host selection and to support diagnostics development.

分析和映射核心 B 项目负责人：Kyu Rhee 和 D. Branch Moody 联合调查员：雅各布·梅菲尔德摘要 - 分析和映射核心 B 现代传染病研究从根本上依赖于主要病原体的基因组图谱。随着最近开发的全生物体代谢组学分析工具，全面绘制了 M. 结核病（Mtb）代谢组现在是可行的。 Core B 支持基于质谱的 Mtb 分析代谢物来支持项目 1、2 和 3，分别侧重于发现、药物和诊断。这 Core 将进行整个生物体的脂质组学和代谢组学分析、存档数据集并使用现代技术生成 Mtb 代谢脂质组图的生物信息方法。地图由结构化列表组成所有代谢物子类的列表、每个类中所有命名代谢物的列表以及所有命名代谢物的链接代谢物质量值和其他生物变量。我们之前生成了 MycoMap 数据库，这是现有的最大的 183 种分枝杆菌代谢物类别图，与约 195,000 个精确质量相关价值观。我们现在将通过添加项目 1、2 和 3 中生成的新发现的脂质来扩展数据库。支持项目 1 和 2，我们将解决 Mycolate 外膜脂质图，它描述了位于宿主-病原体屏障并参与药物渗透的化合物。支持项目1和3，该核心将测量来自南非患者的 Mtb 菌株之间的代谢变异，以生成人类 Mtb 菌株脂质变异数据库。该数据库将描述脂质的流行和变化在人类社区中传播的菌株中识别由宿主选择控制的脂质并支持诊断开发。