Deep brain photoacoustic tomography at single-neuron resolution using arrays of photonic emitters and high-frequency ultrasound transducers

使用光子发射器和高频超声换能器阵列进行单神经元分辨率的深部脑光声断层扫描

• 批准号: 9231961
• 负责人: MICHAEL L ROUKES
• 金额: $ 101.05万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-30 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9231961
• 关键词: Acoustics; Affect; Alzheimer' s Disease; Animals; Area; Blood capillaries; Brain; Brain imaging; Cephalic; Detection; Development; Diffuse; Dura Mater; Electronics; Elements; Event; Experimental Water Deprivation; Foundations; Frequencies; Geometry; Goals; Hemoglobin; Hypothalamic structure; Image; Injection of therapeutic agent; Lasers; Light; Lighting; Maps; Measures; Methods; Microelectrodes; Microscopy; Modality; Modeling; Mus; Neurons; Optics; Parkinson Disease; Pattern; Penetration; Research Project Grants; Resolution; Rewards; Saline; Sampling; Science; Side; Signal Transduction; Speed; System; Technology; Three-Dimensional Imaging; Time; Transducers; Ultrasonic Transducer; Validation; Work; X-Ray Computed Tomography; base; brain tissue; capillary; data acquisition; imaging system; improved; in vivo; mathematical ability; meetings; nervous system disorder; neuroimaging; novel; novel strategies; photonics; prototype; reconstruction; signal processing; tomography; two-photon

The objective of the proposed three-year research project is to develop high-speed, high-spatial- resolution, deep-penetration photoacoustic computed tomography (PACT) for real-time imaging of neuro-activities in mouse brains in vivo. The proposed hardware imaging system will be unprecedented in the field of PACT in terms of its volumetric rate and spatial resolving power, which benefit from the use of the largest ever number of sensing elements with one-to-one mapped digitization channels. In comparison to existing high-resolution optical neuroimaging modalities such as two-photon microscopy, the proposed system will provide deeper penetration, and higher volumetric imaging speed for whole mouse brain imaging. The timing for such an exciting project is perfect because of two events. (1) Our unpublished ongoing work has shown for the first time that PACT has imaged through the entire mouse brain, with abundant vessels resolved (see the images in the Aim 4 section). (2) High-frequency ultrasonic transducer elements with omnidirectional sensitivity can be massively integrated by the Shepard lab to accomplish three-dimensional (3D) acoustic detection (discussed in the Aim 3 section). By using an unprecedented 1536 ultrasonic transducer elements one-to-one mapped to data acquisition channels, we will perform real-time 3D PACT at single neuron resolution and 2 kHz volumetric rate, which has never been achieved before. The specific aims include: 1. Develop implantable photonic probes with arrays of photonic emitters. 2. Develop high-frequency ultrasonic transducer arrays for PACT. 3. Develop deep brain high-frequency PACT. 4. Use PACT to image neuro-activities in mouse brains in vivo.

拟议的三年研究项目的目的是开发高速，高空间 分辨率，深度渗透光声计算机断层扫描（PACT）用于实时成像 在体内小鼠大脑中的神经活性。提议的硬件成像系统将是 就其体积率和空间分辨能力而言，契约领域前所未有 通过使用有史以来最大数量的感应元素的使用，受益于一对一的映射 数字化通道。与现有的高分辨率光学神经影像模式相比 作为两光子显微镜，所提出的系统将提供更深的穿透力，并且更高 整个鼠标脑成像的体积成像速度。 由于两个事件，因此对这样一个激动人心的项目的时机非常完美。 （1）我们未出版 正在进行的工作首次表明，PACT在整个鼠标大脑中成像 随着大量容器的解析（请参见AIM 4部分中的图像）。 （2）高频超声波 Shepard Lab可以大规模整合具有全向灵敏度的传感器元件 完成三维（3D）声学检测（在AIM 3部分中进行了讨论）。通过使用 前所未有的1536个超声传感器元素一对一映射到数据采集 通道，我们将在单个神经元分辨率和2 kHz体积速率下执行实时3D PACT， 这从未有过。 具体目的包括： 1。开发具有光子发射器阵列的可植入光子探针。 2。为条约开发高频超声传感器阵列。 3。建立深层大脑高频协议。 4。使用条约在体内成像小鼠大脑中的神经活性。