Identification of cell-type specific visual circuits in the ventral lateral geniculate nucleus

腹外侧膝状核中细胞类型特异性视觉回路的识别

基本信息

批准号：
10598133
负责人：
John N Campbell
金额：
$ 19.56万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10598133
关键词：
Affect Anatomy Atlases Behavior Brain Brain region Catalogs Cell Nucleus Cells Dorsal Economic Burden Genetic Genetic Transcription Goals Hypothalamic structure Image Immunohistochemistry In Situ Hybridization Interneurons Investigation Knowledge Label Lateral Geniculate Body Link Logic Midbrain structure Molecular Morphology Mus Natural regeneration Neurons Outcome Output Persons Population Process Proteome Reporter Resolution Retina Retinal Ganglion Cells Rodent Signal Transduction Spatial Distribution Stream Subthalamic structure Synapses Testing Thalamic structure Tracer Viral Vision Visual Visual Pathways Visual System Visual impairment cell type circadian cohort effective therapy gaze light transmission novel response retinal axon sight restoration social superior colliculus Corpora quadrigemina suprachiasmatic nucleus tool transcriptome transcriptome sequencing transcriptomics transmission process visual information

项目摘要

PROJECT SUMMARY Information about the visual world is captured by the retina and transmitted by retinal ganglion cells (RGCs) to a diverse array of retino-recipient regions of the mammalian brain, including those in thalamic, hypothalamic, and midbrain regions. There is an organizational logic to these long-range retinal projections where RGCs, of which there are more than three dozen morphologically and functionally distinct subtypes, project to distinct and sometimes mutually exclusive retinorecipient regions. Many of these retinorecipient nuclei are critical to the execution of specific visual behaviors. To understand how visual information is processed and transmitted from the retina to these brain regions requires a detailed characterization of the full cohort of cells in both regions and an investigation into the types of circuits they are assembled into. This requires us to catalogue a cellular “parts list” in both the retina and brain. For some parts of the subcortical visual system, such a catalogue exists. However, for some retino-recipient nuclei the cellular parts list is either poorly characterized or missing. One such region that remains poorly characterized is the ventral lateral geniculate nucleus (vLGN), the third largest retino-recipient region in rodents. Therefore, our goal is to apply state-of-the-art single cell resolution transcriptomic sequencing with trans-synaptic viral tracing to identify, in an unbiased fashion, all types of retino-recipient cells in the vLGN. Two such cell types have been identified in preliminary studies (Pvalb+ and Penk+ cells) and surprisingly, these cell types occupy distinct, adjacent sublamina of vLGN. These anatomical divisions suggest that subtype-specific laminar distribution of retino-recipient cells in this region may be important for receiving, processing, and transmitting light-derived signals in parallel channels through the brain. We will test this hypothesis by identifying the specific inputs and outputs of these two subtypes of vLGN neurons.

项目概要有关视觉世界的信息由视网膜捕获，并由视网膜神经节细胞 (RGC) 传输至哺乳动物大脑中一系列不同的视网膜受体区域，包括丘脑、下丘脑、这些远程视网膜投射有一个组织逻辑，其中 RGC 是其中有超过三打形态和功能不同的亚型，投射到不同的有时，许多视网膜受体区域是相互排斥的。了解特定视觉行为的执行方式。从视网膜到这些大脑区域，需要对两个区域的完整细胞群进行详细表征区域并调查它们组装成的电路类型，这需要我们对它们进行分类。视网膜和大脑中的细胞“部件列表” 对于皮层下视觉系统的某些部分，例如然而，对于某些视网膜受体来说，细胞部件列表的特征很差。腹侧外侧膝状体核（vLGN）是这样一个仍不清楚的区域，因此，我们的目标是应用最先进的单细胞。分辨率转录组测序与跨突触病毒追踪，以公正的方式识别所有初步研究已鉴定出 vLGN 中的两种视网膜受体细胞类型。（Pvalb+ 和 Penk+ 细胞）令人惊讶的是，这些细胞类型占据了 vLGN 不同的、相邻的亚层。解剖学划分表明该区域视网膜受体细胞的亚型特异性层状分布对于通过并行通道接收、处理和传输光衍生信号可能很重要我们将通过识别这两种亚型的具体输入和输出来检验这个假设。 vLGN 神经元。