Neuropathology Core

神经病理学核心

• 批准号: 8838023
• 负责人: DENNIS WILLIAM DICKSON
• 金额: $ 19.72万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8838023
• 关键词: Aging; Alzheimer' s Disease; Amyloid beta-Protein; Animal Model; Antibodies; Autopsy; Biometry; Biostatistics Core; Brain; Cerebral Amyloid Angiopathy; Classification; Clinic; Clinical; Clinical Services; Computer software; Consensus; Correlative Study; DNA; Data; Databases; Dementia; Diagnosis; Diagnostic; Dissection; Educational Activities; Educational workshop; Elderly; Electronics; Enrollment; Evaluation; Frontotemporal Lobar Degenerations; Funding; Genetic; Genetic Screening; Genetic study; Gold; Hippocampus (Brain); Histologic; Image; Immunohistochemistry; Individual; Institution; Lead; Leadership; Lewy Bodies; Lewy Body Dementia; Longitudinal Studies; MRI Scans; Magnetic Resonance Imaging; Maps; Measures; Methods; Microscopy; Mission; Multicenter Studies; Neurofibrillary Tangles; Participant; Pathologic; Pathology; Patients; Pilot Projects; Play; Protocols documentation; Qualifying; Quality Control; Quantitative Evaluations; Research; Research Personnel; Research Priority; Research Project Grants; Role; Sampling; Schedule; Sclerosis; Services; Severities; Shapes; Silver Staining; Tauopathies; Thioflavin S; Tissue Sample; Tissues; United States National Institutes of Health; Videoconferences; Videoconferencing; alpha synuclein; base; brain tissue; cerebrovascular; cerebrovascular lesion; data management; gene discovery; human tissue; improved; mild cognitive impairment; mouse model; nervous system disorder; neuroimaging; neuropathology; non-alzheimer dementia; pre-clinical; programs; protein TDP-43; research study; screening; tau Proteins; tissue processing

The Neuropathology (NP) Core performs diagnostic evaluations and quantitative analyses on brain tissue collected at autopsy of participants in the Mayo Alzheimer Disease Research Center (ADRC). The NP Core provides support to research projects of the ADRC and to other center affiliated investigators. The neuropathologic and genetic data generated by the NP Core is communicated to the Biostatistics and Data Management Core and to the National Alzheimer Coordinating Center. The approach for the NP Core is to: 1. Perform brain autopsies on participants of the Mayo ADRC in a timely fashion and according to protocol. 2. Provide neuropathologic evaluations and collect neuropathologic data using standardized methods for gross dissection and neurohistology. a. Cooperate with Mayo Clinic research neuroradiology in acquisition of post-mortem MRI scans of brains. b. Dissect brains according to a standardized protocol and photograph all sections. Score the severity of cerebrovascular pathology, and measure and sample macroscopic cerebrovascular lesions. c. Use histological methods, including silver stains, thioflavin S fluorescent microscopy and immunohistochemistry for tau and Aβ, to collect standardized pathologic data on all cases. d. Perform immunostaining with antibodies to α-synuclein on all cases. In cases with Lewy bodies, assign a diagnosis according to the criteria of the Consortium for Dementia with Lewy Bodies. e. Perform immunostaining with antibodies to TDP-43 on all cases and when positive on the screening section, subtype and map the distribution of TDP-43 pathology. f. Arrive at a consensus on clinicopathologic diagnoses at videoconferences held twice a month. g. Provide neuropathological data to the Biostatistics Core and NACC. 3. Store brain tissue and other autopsy-derived materials (e.g., DNA) and provide clinically and pathologically well-characterized tissue samples, DNA or data to ADRC research projects and pilot projects at Mayo Clinic as well as qualified outside investigators. 4. Assist ADRC research projects by providing neuropathologic expertise, tissue or histopathologic services. a. Assist in neuropathologic classification of cases included in the MRI studies of Project 1, which aims to develop an image based diagnostic software program that predicts the underlying pathology. b. Provide autopsy tissue samples of brains of subjects with a range of neurofibrillary pathology in AD and other tauopathies to Project 2 and assist in tissue processing and histologic studies of mouse models. c. Provide brain tissue samples to Project 3 as well as data on cerebral amyloid angiopathy (CAA) with respect to type, distribution, and severity; as well as data on burden of cerebrovascular pathology. 5. Provide genetic screening for ADRC sample sets with respect to existing and newly discovered genes.

神经病理学（NP）核心对脑组织进行诊断评估和定量分析 在阿尔茨海默氏病研究中心（ADRC）的参与者的尸检中收集。 NP核心 为ADRC的研究项目和其他中心分支机构研究人员提供支持。这 NP核心产生的神经病理学和遗传数据已传达给生物统计学和数据 管理核心和国家阿尔茨海默氏症协调中心。 NP核心的方法是： 1。根据协议，对Mayo ADRC的参与者进行大脑尸检。 2。提供神经病理学评估并使用标准化方法来收集神经病理学数据 总的解剖和神经组织学。 一个。与Mayo诊所研究神经放射学合作，以获取大脑后MRI扫描。 b。根据标准化协议剖析大脑，并拍摄所有部分。评分 脑血管病理学，测量和样品宏观脑血管病变。 c。使用组织学方法，包括银色污渍，硫诺夫蛋白的荧光显微镜和 TAU和Aβ的免疫组织化学在所有情况下收集标准化的病理数据。 d。在所有情况下，用抗α-突触核蛋白的抗体进行免疫染色。在有路易尸体的情况下，分配 根据Lewy Bodies的痴呆症联盟的标准，​​诊断。 e。在所有情况下，用抗TDP-43抗体进行免疫染色 部分，亚型和映射TDP-43病理的分布。 f。在每月两次举行的视频会议上达成了对临床病理诊断的共识。 g。向生物病理学核心和NACC提供神经病理学数据。 3。存储脑组织和其他尸检材料（例如DNA），并在临床和病理上提供 梅奥（Mayo）的ADRC研究项目和试点项目的特征良好的组织样品，DNA或数据 诊所以及合格的外部研究人员。 4。通过提供神经病理专业知识，组织或组织病理学服务来协助ADRC研究项目。 一个。协助项目1 MRI研究中包括的病例的神经病理学分类，该研究旨在 开发基于图像的诊断软件程序，以预测潜在的病理。 b。在AD和 其他tauopathies旨在投影2并有助于小鼠模型的组织加工和组织学研究。 c。为项目3提供脑组织样品以及有关脑淀粉样血管病（CAA）的数据 尊重类型，分布和严重性；以及有关脑血管病理燃烧的数据。 5。相对于现有和新发现的基因提供ADRC样品集的遗传筛查。