Neuropathology Core

神经病理学核心

基本信息

批准号：
10657557
负责人：
DENNIS WILLIAM DICKSON
金额：
$ 24.07万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10657557
关键词：
Aging Alzheimer&apos s Disease Alzheimer&apos s disease related dementia Amygdaloid structure Amyloid Amyloid beta-Protein Area Autopsy Biological Biological Assay Brain C9ORF72 Cerebellum Clinic Clinical Clinical Medicine Collection Communication Consensus Correlative Study DNA Data Dedications Dementia Dementia with Lewy Bodies Diagnosis Diagnostic Dissection Educational Activities Educational workshop Electronic Mail Electronics Emergency Situation Evaluation Fostering Freezing Frontotemporal Lobar Degenerations Funding Genetic Genetic Risk Genotype Guidelines Hippocampus Histologic Human Resources Image Analysis Immunohistochemistry Institution LRRK2 gene Lewy Body Disease Lewy Body Variant of Alzheimer&apos s Disease Maps Measures Methods Mission Monitor Multicenter Studies National Institute on Alcohol Abuse and Alcoholism Neurodegenerative Disorders Neurofibrillary Tangles Participant Pathologic Pathology Phase Play Probability Protocols documentation Qualifying Quality Control Research Research Personnel Role Safety Sampling Scheme Scientist Security Senile Plaques Severities Shapes Standardization TREM2 gene Tauopathies Tissue Sample Tissues United States National Institutes of Health age related age related neurodegeneration alpha synuclein brain tissue cerebrovascular lesion cerebrovascular pathology data management density digital imaging digital pathology endophenotype gene discovery genetic information genetic testing genetic variant improved molecular imaging neuroimaging neuropathology novel pre-clinical presenilin-1 protein TDP-43 screening statistics tau Proteins videoconference

项目摘要

PROJECT DESCRIPTION/ABSTRACT – NEUROPATHOLOGY CORE The Neuropathology (NP) Core performs diagnostic evaluations, quantitative analyses on brain tissue collected at autopsy of participants in the Mayo Alzheimer Disease Research Center (ADRC), and genetic testing of known and novel genetic variants. The NP Core provides support to ADRC-affiliated investigators and to outside investigators. The neuropathologic and genetic data generated by the NP Core is communicated to the Data Management and Statistical Core, who uploads it to the National Alzheimer Coordinating Center. The research approach for the NP Core is as follows: Specific Aim 1. Perform brain autopsies on participants of the Mayo ADRC in a timely fashion and according to protocol, which includes collection of fixed and frozen tissue. Specific Aim 2. Provide neuropathologic evaluations and collect neuropathologic data using standardized methods for gross dissection and neurohistology. Assign AD likelihood diagnoses based upon Thal amyloid phase, Braak NFT stage, and CERAD neuritic plaque score using the NIA-AA diagnostic probability scheme. Arrive at a consensus on clinicopathologic diagnoses at videoconferences. Record neuropathologic data on NACC Version 10 and provide to the Data Management and Statistical Core and NACC. Specific Aim 3. Generate quantitative endophenotypes using digital pathology and image analysis in support of ADRC research. Specific Aim 4. Store brain tissue and other autopsy-derived materials and provide clinically and pathologically well-characterized tissue samples to investigators at Mayo Clinic and outside institutions. Assure security of biologic samples by using continuous electronic monitoring of freezers and by maintaining at all times at least one empty back-up freezer. Specific Aim 5. Provide genetic expertise to ADRC, as well as screening for known and newly discovered genes or common genetic risk variants for AD, Lewy body disorders (LBD) and frontotemporal lobar degenerations (FTLD).

项目描述/摘要 – 神经病理学核心神经病理学 (NP) 核心对脑组织进行诊断评估和定量分析梅奥阿尔茨海默病研究中心 (ADRC) 对参与者进行尸检时收集的信息，以及遗传信息 NP 核心为 ADRC 附属研究人员提供支持。以及 NP Core 生成的神经病理学和遗传数据。传达给数据管理和统计核心，后者将其上传到国家阿尔茨海默病中心协调中心 NP Core 的研究方法如下：具体目标 1. 执行大脑根据协议及时对梅奥 ADRC 参与者进行尸检，其中包括收集固定和冷冻的组织。具体目标 2. 提供神经病理学评估并收集。使用标准化方法进行神经病理学数据的大体解剖和神经组织学分配。基于 Thal 淀粉样蛋白阶段、Braak NFT 阶段和 CERAD 神经炎斑块评分的可能性诊断使用 NIA-AA 诊断概率方案达成临床病理诊断共识。记录 NACC 版本 10 上的神经病理学数据并提供给数据管理部门统计核心和 NACC 具体目标 3. 使用数字生成定量内表型。支持 ADRC 研究的病理学和图像分析。具体目标 4. 储存脑组织和其他组织。尸检材料并提供临床和病理学特征良好的组织样本梅奥诊所和外部机构的研究人员通过连续使用确保生物样本的安全。对冰柜进行电子监控，并始终保持至少一台空的备用冰柜。具体目标 5. 向 ADRC 提供遗传专业知识，并筛选已知和新发现的 AD、路易体疾病 (LBD) 和额颞叶的基因或常见遗传风险变异变性（FTLD）。