Flow Cytometry

流式细胞仪

• 批准号: 10617298
• 负责人: Michael Birnbaum
• 金额: $ 23.25万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-17 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10617298
• 关键词: Antibodies; Apoptosis; Bioinformatics; Budgets; Cancer Center Support Grant; Cell Cycle Progression; Cell Separation; Cell surface; Cells; Collaborations; Consultations; Cytometry; DNA Damage; Data Analyses; Detection; Development; Education; Educational workshop; Engineering; Evaluation; Experimental Designs; Flow Cytometry; Fluorescence-Activated Cell Sorting; Funding; Genomics; Imaging technology; Individual; Information Centers; Label; Lasers; Methods; Mission; Multiplexed Ion Beam Imaging; Phenotype; Population; Proteins; Protocols documentation; Recovery; Reporter Genes; Research; Research Personnel; Research Support; Resource Sharing; Resources; Robotics; Sampling; Services; Source; Specialist; Speed; System; Technical Expertise; Techniques; Technology; Therapeutic; Training; anticancer research; copolymer; data acquisition; fluorophore; instrument; instrumentation; interest; member; operation; programs; response; single cell analysis; single cell mRNA sequencing; single-cell RNA sequencing; success; training opportunity

Flow Cytometry Core: Project Summary/Abstract Flow cytometry is an essential method in cancer research. It is widely used to identify, track and purify specific cell populations through analysis of cell surface markers and/or fluorescent reporter genes, and also to assess cellular phenotypes, such as cell cycle progression, DNA damage or apoptosis responses. Flow cytometry also supports a broad range of engineering-based approaches, such as quantification of targeting efficiencies for antibody- and copolymer-based therapeutic delivery systems, and high-throughput approaches to develop fluorophore-based protein labeling and imaging technology. The Koch Institute Flow Cytometry Core is a Shared Resource that provides Center Members with training in, and access to, state-of-the-art cell sorting instrumentation, technical expertise and training, and data analyses. In the current period, the capabilities of this Core were further expanded and enhanced. Two new analyzers and two new sorters were acquired, replacing older obsolete instrumentation, and three systems were upgraded with new lasers to expand detection capabilities. Core service usage by Center Member has remained high (93% of Center Members). In addition, using CCSG Developmental Funds, the Core has supported a Research Specialist who has established a suite of single cell-associated technologies, including a collaboration with the Koch Institute Integrated Genomics & Bioinformatics Core to enable high throughput single cell mRNA sequencing. These services are now well-developed services within the Core, and we seek continued, partial funding to support the Research Specialist’s efforts. In the upcoming period, the Flow Cytometry Core will continue to offer a wide range of state-of-the-art services to support the research programs of Center Members and will evaluate emerging capabilities in the context of Center Member needs and interests. The Core intends to evaluate high parameter cell sorting, addition of robotics capabilities for automated sampling, mass cytometry, and multiplexed ion beam imaging. The Core will also expand training opportunities, including hands-on data analysis workshops. This Shared Resource is essential to the success of the Koch Institute mission and provides exceptional value to the CCSG because Koch Institute Members account for 84% of the Core services usage. The requested CCSG budget for Year 49 is increased by 9.2% over the budget in the current period (Year 48), reflecting the request for additional funding to stabilize support for the new single cell analysis capabilities.

流式细胞术核心：项目摘要/摘要 流式细胞术是癌症研究中的重要方法，广泛用于识别、跟踪和纯化特异性。 通过分析细胞表面标记和/或荧光报告基因来分析细胞群，并评估 细胞表型，例如细胞周期进展、DNA 损伤或细胞凋亡反应。 支持广泛的基于工程的方法，例如量化目标效率 基于抗体和共聚物的治疗递送系统，以及开发高通量方法 基于荧光团的蛋白质标记和成像技术是科赫研究所流式细胞术核心。 共享资源为中心成员提供最先进的细胞分选培训和使用机会 仪器仪表、技术专业知识和培训以及数据分析。 当前，该核心的功能得到了进一步扩展和增强，两款新分析仪。 购买了两台新的分拣机，取代了旧的过时仪器，并安装了三个系统 升级了新的激光器，以扩展中心成员的核心服务使用。 仍然很高（93% 的中心成员）。此外，利用 CCSG 发展基金，核心已实现了这一目标。 支持一位研究专家建立了一套单细胞相关技术，包括 与科赫研究所综合基因组学和生物信息学核心合作以实现高通量 这些服务现在是核心内成熟的服务，我们寻求。 持续提供部分资金支持研究专家的工作。 在接下来的一段时间内，流式细胞术核心将继续提供广泛的最先进的服务 支持中心成员的研究计划，并将评估新兴能力 中心成员的需求和兴趣。核心打算评估高参数细胞分选，添加 用于自动采样、质谱流式分析和多重离子束成像的机器人功能。 还将扩大培训机会，包括实践数据分析研讨会。 对于科赫研究所使命的成功至关重要，并且为 CCSG 提供了非凡的价值，因为 科赫研究所成员占 49 年核心服务使用请求的 CCSG 预算的 84%。 比本期（第 48 年）预算增加 9.2%，反映了追加预算的要求 资金支持稳定新的单细胞分析能力。